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Long Chain Polyunsaturated Fatty acids in Granulocytes, Mononuclear Cells and Red Blood Cells in Patients with Cystic Fibrosis: Relation to Liver Disease.
MedLine Citation:
PMID:  22241510     Owner:  NLM     Status:  Publisher    
BACKGROUND:: Patients with cystic fibrosis (CF) have low levels of n-3 long chain polyunsaturated fatty acids (n-3 LCPUFA) in plasma or red blood cells (RBC), as also seen in other chronic and acute liver diseases. The differences may be more pronounced in cystic fibrosis transmembrane conductance regulator protein (CFTR) regulated tissues such as granulocytes, monocytes and lymfocytes. AIM:: To investigate if patients with CF-related liver disease (CF-RL) have lower n-3 LCPUFA level than patients with CF without liver disease. METHODS:: Twenty patients with known CF-RL were matched with 20 CF patients without. Blood samples were analysed for liver biochemistry and haematology. Granulocytes, mononuclear cells and RBC were separated by density gradient centrifugation and fatty acid composition was measured by gas chromatography. Hepatic ultrasound was scored according to Williams et al. (WUSS). Hepatic transit time (HTT) was measured with the ultrasound contrast agent SONOVUE (Bracco). RESULTS:: No significant differences were seen in either n-6 or n-3 LCPUFAs in any cell line when the two groups were compared. In a multiple regression analysis including HTT, age, P.Aeg infection, diabetes mellitus, treatment with ursodeoxycholic acid, FEV1 (% of predicted value) and WUSS, only n-3 LCPUFA docosaehexanoic acid (DHA) in mononuclear cell membranes was positively associated with HTT (p = 0.02). The AA/DHA ratio within the mononuclear cells was both negatively associated with HTT (P = 0.003) and WUSS (p = 0.03). For RBC-LCPUFAs no significant associations were seen. CONCLUSION:: These findings indicate that in CF patients, the degree of liver disease was negatively associated with LCPUFA n-3 levels in CFTR expressing white blood cells but unrelated to those levels in CFTR negative RBC.
Marianne Hørby Jørgensen; Peter Ott; Kim F Michaelsen; Trine Porsgaard; Fleming Jensen; Susanne Lanng
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-1-10
Journal Detail:
Title:  Journal of pediatric gastroenterology and nutrition     Volume:  -     ISSN:  1536-4801     ISO Abbreviation:  -     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-1-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8211545     Medline TA:  J Pediatr Gastroenterol Nutr     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
*Department of Paediatrics, Rigshospitalet, Copenhagen, Denmark †Department of Hepatology and Gastroenterology, Aarhus university hospital, Aarhus, Denmark ‡Department of Human Nutrition, Faculty of Life Sciences, University of Copenhagen,, Frederiksberg, Denmark §Biocentrum-DTU, Technical University of Denmark, Lyngby ||Ultrasound section, Rigshospitalet, Copenhagen, Denmark ¶Department of pediatrics, KAS Glostrup, Glostrup Denmark.
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