| Long astral microtubules and RACK-1 stabilize polarity domains during maintenance phase in Caenorhabditis elegans embryos. | |
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MedLine Citation:
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PMID: 21533050 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cell polarity is a very well conserved process important for cell differentiation, cell migration, and embryonic development. After the establishment of distinct cortical domains, polarity cues have to be stabilized and maintained within a fluid and dynamic membrane to achieve proper cell asymmetry. Microtubules have long been thought to deliver the signals required to polarize a cell. While previous studies suggest that microtubules play a key role in the establishment of polarity, the requirement of microtubules during maintenance phase remains unclear. In this study, we show that depletion of Caenorhabditis elegans RACK-1, which leads to short astral microtubules during prometaphase, specifically affects maintenance of cortical PAR domains and Dynamin localization. We then investigated the consequence of knocking down other factors that also abolish astral microtubule elongation during polarity maintenance phase. We found a correlation between short astral microtubules and the instability of PAR-6 and PAR-2 domains during maintenance phase. Our data support a necessary role for astral microtubules in the maintenance phase of cell polarity. |
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Authors:
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Erkang Ai; Daniel S Poole; Ahna R Skop |
Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. Date: 2011-04-20 |
Journal Detail:
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Title: PloS one Volume: 6 ISSN: 1932-6203 ISO Abbreviation: PLoS ONE Publication Date: 2011 |
Date Detail:
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Created Date: 2011-05-02 Completed Date: 2011-08-30 Revised Date: 2013-04-17 |
Medline Journal Info:
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Nlm Unique ID: 101285081 Medline TA: PLoS One Country: United States |
Other Details:
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Languages: eng Pagination: e19020 Citation Subset: IM |
Affiliation:
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Laboratory of Genetics, University of Wisconsin-Madison, Madison, Wisconsin, United States of America. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Body Patterning* Caenorhabditis elegans / embryology*, metabolism Caenorhabditis elegans Proteins / metabolism, physiology* Cell Polarity / physiology* Dynamins / metabolism Fluorescence Metaphase Microtubules / physiology* RNA Interference Receptors, Cytoplasmic and Nuclear / physiology* |
| Grant Support | |
ID/Acronym/Agency:
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K01 HL092583/HL/NHLBI NIH HHS; K01 HL092583-01/HL/NHLBI NIH HHS; K01 HL092583-02/HL/NHLBI NIH HHS; K01 HL092583-03/HL/NHLBI NIH HHS; K01 HL092583-05/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Caenorhabditis elegans Proteins; 0/RACK-1 protein, C elegans; 0/Receptors, Cytoplasmic and Nuclear; EC 3.6.5.5/Dyn-1 protein, C elegans; EC 3.6.5.5/Dynamins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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