Document Detail


Long astral microtubules and RACK-1 stabilize polarity domains during maintenance phase in Caenorhabditis elegans embryos.
MedLine Citation:
PMID:  21533050     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cell polarity is a very well conserved process important for cell differentiation, cell migration, and embryonic development. After the establishment of distinct cortical domains, polarity cues have to be stabilized and maintained within a fluid and dynamic membrane to achieve proper cell asymmetry. Microtubules have long been thought to deliver the signals required to polarize a cell. While previous studies suggest that microtubules play a key role in the establishment of polarity, the requirement of microtubules during maintenance phase remains unclear. In this study, we show that depletion of Caenorhabditis elegans RACK-1, which leads to short astral microtubules during prometaphase, specifically affects maintenance of cortical PAR domains and Dynamin localization. We then investigated the consequence of knocking down other factors that also abolish astral microtubule elongation during polarity maintenance phase. We found a correlation between short astral microtubules and the instability of PAR-6 and PAR-2 domains during maintenance phase. Our data support a necessary role for astral microtubules in the maintenance phase of cell polarity.
Authors:
Erkang Ai; Daniel S Poole; Ahna R Skop
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2011-04-20
Journal Detail:
Title:  PloS one     Volume:  6     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2011  
Date Detail:
Created Date:  2011-05-02     Completed Date:  2011-08-30     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e19020     Citation Subset:  IM    
Affiliation:
Laboratory of Genetics, University of Wisconsin-Madison, Madison, Wisconsin, United States of America.
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MeSH Terms
Descriptor/Qualifier:
Animals
Body Patterning*
Caenorhabditis elegans / embryology*,  metabolism
Caenorhabditis elegans Proteins / metabolism,  physiology*
Cell Polarity / physiology*
Dynamins / metabolism
Fluorescence
Metaphase
Microtubules / physiology*
RNA Interference
Receptors, Cytoplasmic and Nuclear / physiology*
Grant Support
ID/Acronym/Agency:
K01 HL092583/HL/NHLBI NIH HHS; K01 HL092583-01/HL/NHLBI NIH HHS; K01 HL092583-02/HL/NHLBI NIH HHS; K01 HL092583-03/HL/NHLBI NIH HHS; K01 HL092583-05/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Caenorhabditis elegans Proteins; 0/RACK-1 protein, C elegans; 0/Receptors, Cytoplasmic and Nuclear; EC 3.6.5.5/Dyn-1 protein, C elegans; EC 3.6.5.5/Dynamins
Comments/Corrections

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