| Lomustine Loaded Chitosan Nanoparticles: Characterization and in-Vitro Cytotoxicity on Human Lung Cancer Cell Line L132. | |
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MedLine Citation:
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PMID: 21372411 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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The aim of this work was to prepare chitosan nanoparticles loaded with antineoplastic drug Lomustine (LCNPs), by ionic-gelation method with homogenization. The nanoparticles were characterized for particle size, polydispersity index (PDI), surface morphology, encapsulation efficiency, in-vitro drug release and cytotoxicity on human lung cancer cell line L132 by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The particle size, zeta potential and encapsulation efficiency of prepared nanoparticles ranged from 75±1.1 to 637±1.6 nm (PDI from 0.05±0.001 to 0.18±0.007), 37.2±0.21 to 53.8±0.18 mV and 66.74±1.4 to 98.0±1.8% respectively. The particles were spherical in shape with smooth surface in scanning electron microscopy (SEM) and transmission electron microscopy (TEM) images. Mechanical shearing by homogenization treatment significantly changed the nanoparticle size. The drug release rate was biphasic and diffusion controlled over the 8 h. LCNPs greatly inhibited the growth of the L132 cancer cell line used in this study in comparison to the native Lomustine (LMT). |
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Authors:
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Archana Mehrotra; Ramesh Chand Nagarwal; Jayanta Kumar Pandit |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Chemical & pharmaceutical bulletin Volume: 59 ISSN: 1347-5223 ISO Abbreviation: Chem. Pharm. Bull. Publication Date: 2011 |
Date Detail:
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Created Date: 2011-03-04 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0377775 Medline TA: Chem Pharm Bull (Tokyo) Country: Japan |
Other Details:
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Languages: eng Pagination: 315-20 Citation Subset: IM |
Affiliation:
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NRI Institute of Pharmaceutical Sciences. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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