| Logic gates and antisense DNA devices operating on a translator nucleic Acid scaffold. | |
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MedLine Citation:
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PMID: 19507821 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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A series of logic gates, "AND", "OR", and "XOR", are designed using a DNA scaffold that includes four "footholds" on which the logic operations are activated. Two of the footholds represent input-recognition strands, and these are blocked by complementary nucleic acids, whereas the other two footholds are blocked by nucleic acids that include the horseradish peroxidase (HRP)-mimicking DNAzyme sequence. The logic gates are activated by either nucleic acid inputs that hybridize to the respective "footholds", or by low-molecular-weight inputs (adenosine monophosphate or cocaine) that yield the respective aptamer-substrate complexes. This results in the respective translocation of the blocking nucleic acids to the footholds carrying the HRP-mimicking DNAzyme sequence, and the concomitant release of the respective DNAzyme. The released product-strands then self-assemble into the hemin/G-quadruplex-HRP-mimicking DNAzyme that biocatalyzes the formation of a colored product and provides an output signal for the different logic gates. The principle of the logic operation is, then, implemented as a possible paradigm for future nanomedicine. The nucleic acid inputs that bind to the blocked footholds result in the translocation of the blocking nucleic acids to the respective footholds carrying the antithrombin aptamer. The released aptamer inhibits, then, the hydrolytic activity of thrombin. The system demonstrates the regulation of a biocatalytic reaction by a translator system activated on a DNA scaffold. |
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Authors:
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Bella Shlyahovsky; Yang Li; Oleg Lioubashevski; Johann Elbaz; Itamar Willner |
Publication Detail:
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Type: Journal Article Date: 2009-06-09 |
Journal Detail:
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Title: ACS nano Volume: 3 ISSN: 1936-086X ISO Abbreviation: ACS Nano Publication Date: 2009 Jul |
Date Detail:
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Created Date: 2011-04-01 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101313589 Medline TA: ACS Nano Country: United States |
Other Details:
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Languages: eng Pagination: 1831-43 Citation Subset: - |
Affiliation:
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Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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