Document Detail


Locomotor activity level monitoring using the Drosophila Activity Monitoring (DAM) System.
MedLine Citation:
PMID:  21041391     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Adult behavioral assays have been used with great success in Drosophila melanogaster to identify circadian rhythm genes. In particular, the locomotor activity assay can identify altered behavior patterns over the course of several days in small populations, or even individual flies. Commercially available, highly efficient automated systems allow for continuous data collection from large numbers of individuals, and analytical tools make it possible to quickly analyze multiple aspects of circadian behavior from each experiment. These features make the locomotor activity assay useful for high-throughput analyses, leading to the rapid discovery and functional characterization of many Drosophila circadian rhythm genes. The locomotor assay described here can simultaneously assess both circadian and sleep behavior, and several methods can be used to analyze the data generated from such assays. This protocol details the use of the Drosophila Activity Monitoring (DAM) System from TriKinetics. Briefly, the system records activity from individual flies maintained in sealed tubes placed in activity monitors. An infrared beam directed through the midpoint of each tube measures an "activity event" each time a fly crosses the beam. Events detected over the course of each consecutive sampling interval are summed and recorded over the course of the experiment for each fly. The general approaches described here can be applied to a wide range of behavioral activity experiments, including sleep deprivation analyses and general studies of hypoactivity and hyperactivity.
Authors:
Cory Pfeiffenberger; Bridget C Lear; Kevin P Keegan; Ravi Allada
Publication Detail:
Type:  Journal Article     Date:  2010-11-01
Journal Detail:
Title:  Cold Spring Harbor protocols     Volume:  2010     ISSN:  1559-6095     ISO Abbreviation:  Cold Spring Harb Protoc     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-11-02     Completed Date:  2011-05-13     Revised Date:  2014-09-20    
Medline Journal Info:
Nlm Unique ID:  101524530     Medline TA:  Cold Spring Harb Protoc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  pdb.prot5518     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Drosophila melanogaster / physiology*
Entomology / methods*
Motor Activity
Grant Support
ID/Acronym/Agency:
R01 NS059042/NS/NINDS NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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