| Locked nucleic acid: a potent nucleic acid analog in therapeutics and biotechnology. | |
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MedLine Citation:
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PMID: 15294076 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Locked nucleic acid (LNA) is a class of nucleic acid analogs possessing very high affinity and excellent specificity toward complementary DNA and RNA, and LNA oligonucleotides have been applied as antisense molecules both in vitro and in vivo. In this review, we briefly describe the basic physiochemical properties of LNA and some of the difficulties that may be encountered when applying LNA technology. The central part of the review focuses on the use of LNA molecules in regulation of gene expression, including delivery to cells, stability, unspecific effects, toxicity, pharmacokinetics, and design of LNA oligonucleotides. The last part evaluates LNA as a diagnostic tool in genotyping. |
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Authors:
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Jan Stenvang Jepsen; Mads D Sørensen; Jesper Wengel |
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Publication Detail:
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Type: Journal Article; Review |
Journal Detail:
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Title: Oligonucleotides Volume: 14 ISSN: 1545-4576 ISO Abbreviation: Oligonucleotides Publication Date: 2004 |
Date Detail:
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Created Date: 2004-08-05 Completed Date: 2005-11-04 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 101188415 Medline TA: Oligonucleotides Country: United States |
Other Details:
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Languages: eng Pagination: 130-46 Citation Subset: IM |
Affiliation:
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Department of Tumor Endocrinology, Institute of Cancer Biology, Danish Cancer Society, DK-2100, Copenhagen, Denmark. jsj@cancer.dk |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biotechnology / methods* Drug Design Humans Oligonucleotides Oligonucleotides, Antisense / genetics, metabolism, pharmacology*, therapeutic use* Ribonuclease H / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Oligonucleotides; 0/Oligonucleotides, Antisense; 0/locked nucleic acid; EC 3.1.26.4/Ribonuclease H |
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