Document Detail


Locked nucleic acid nucleoside triphosphates and polymerases: on the way towards evolution of LNA aptamers.
MedLine Citation:
PMID:  19603111     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Among numerous nucleic acid analogs reported in the past decades, locked nucleic acid (LNA) has received substantial attention and has become a significant tool within chemical biology disciplines like molecular biology research, diagnostics and therapeutic development. However, despite their obvious structurally unique properties, LNA-based aptamers for diagnostic and therapeutic applications remain largely unexplored. Future evolution of LNA oligonucleotide aptamers will depend on scientific breakthroughs relating to enzymatic polymerization using LNA nucleoside triphosphates as substrates. Herein, we highlight recent developments in this direction using various polymerases.
Authors:
Rakesh N Veedu; Jesper Wengel
Publication Detail:
Type:  Journal Article; Review     Date:  2009-05-15
Journal Detail:
Title:  Molecular bioSystems     Volume:  5     ISSN:  1742-2051     ISO Abbreviation:  Mol Biosyst     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-07-15     Completed Date:  2009-09-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101251620     Medline TA:  Mol Biosyst     Country:  England    
Other Details:
Languages:  eng     Pagination:  787-92     Citation Subset:  IM    
Affiliation:
Department of Physics and Chemistry, Nucleic Acid Center, University of Southern Denmark, Campusvej 55, Odense M, Denmark.
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MeSH Terms
Descriptor/Qualifier:
Aptamers, Nucleotide / chemical synthesis,  chemistry,  metabolism*
DNA-Directed RNA Polymerases / metabolism*
Models, Biological
Oligonucleotides / chemical synthesis,  chemistry,  metabolism*
Chemical
Reg. No./Substance:
0/Aptamers, Nucleotide; 0/Oligonucleotides; 0/locked nucleic acid; EC 2.7.7.6/DNA-Directed RNA Polymerases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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