| Location of intrinsic and inducible phenoloxidase activity in molluscan hemocyanin. | |
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MedLine Citation:
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PMID: 16904637 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The phenoloxidase (PO) activity of the hemocyanins (Hcs) from two molluscan species, the gastropod Helix pomatia (Hp) and the cephalopod Sepia officinalis (So), was studied. With catechol as substrate the Hcs showed a weak o-diPO activity, which was moderately enhanced on limited proteolysis with subtilisin. The sites in the Hc molecules mainly responsible for this activity were identified. The highest intrinsic o-diPO activity and also by far the highest level of induction were found in the functional units (FUs) Hp f and So g, isolated from Hp beta-Hc and So Hc (subunit 2), respectively. The results thus support the earlier conclusion, made on the basis of sequence homology between molluscan Hcs, that Hp f and So g are functional and structural analogues. The subtilisin treatment of Hp f also induced monoPO activity, considered to be at the origin of browning of the sample. |
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Authors:
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Nurul Islam Siddiqui; Roland Forben Akosung; Constant Gielens |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-08-04 |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 348 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2006 Sep |
Date Detail:
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Created Date: 2006-08-22 Completed Date: 2006-10-24 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 1138-44 Citation Subset: IM |
Affiliation:
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Laboratory of Biochemistry, Chemistry Department, Katholieke Universiteit Leuven, Celestijnenlaan 200 G, 3001 Leuven-Heverlee, Belgium. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Catalysis Enzyme Activation Enzyme Induction Helix (Snails) / enzymology* Hemocyanin / chemistry*, metabolism Monophenol Monooxygenase / biosynthesis*, metabolism Oxidation-Reduction Protein Structure, Tertiary Sepia / enzymology* |
| Chemical | |
Reg. No./Substance:
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9013-72-3/Hemocyanin; EC 1.14.18.1/Monophenol Monooxygenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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