| Location of the active site of rat vascular semicarbazide-sensitive amine oxidase. | |
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MedLine Citation:
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PMID: 7931262 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Semicarbazide-sensitive amine oxidase (SSAO) activity in rat vascular smooth muscle cells is associated extensively with the plasmalemma. To determine which side of the plasmalemma the active sites of these enzymes face, the non-permeating agent, diazotised sulphanilic acid (DSA; 4.4 mM) was perfused through the isolated mesenteric arterial bed of the rat, in an attempt to inactivate only those active sites facing extracellularly. DSA perfusion abolished the pressor responses to noradrenaline via inactivation of extracellular alpha 1 receptors but had no effect on cytosolic lactate dehydrogenase activity. SSAO activity, estimated by perfusing [14C] benzylamine, was reduced following DSA perfusion to 55.9 +/- 4.9% of that in control beds and to 52.4 +/- 6.0% in homogenates of these vessels. These results suggest that almost half of SSAO active sites in rat mesenteric arteries face outwards. |
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Authors:
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A Holt; B A Callingham |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of neural transmission. Supplementum Volume: 41 ISSN: 0303-6995 ISO Abbreviation: J. Neural Transm. Suppl. Publication Date: 1994 |
Date Detail:
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Created Date: 1994-11-23 Completed Date: 1994-11-23 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0425126 Medline TA: J Neural Transm Suppl Country: AUSTRIA |
Other Details:
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Languages: eng Pagination: 433-7 Citation Subset: IM |
Affiliation:
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Department of Pharmacology, University of Cambridge, United Kingdom. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amine Oxidase (Copper-Containing)* Animals Azo Compounds / pharmacology* Benzylamines / metabolism Cell Membrane / enzymology Male Muscle, Smooth, Vascular / enzymology* Oxidoreductases Acting on CH-NH Group Donors / metabolism* Rats Rats, Wistar Semicarbazides / pharmacology* Splanchnic Circulation Sulfanilic Acids / pharmacology* Tissue Distribution |
| Chemical | |
Reg. No./Substance:
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0/Azo Compounds; 0/Benzylamines; 0/Semicarbazides; 0/Sulfanilic Acids; 100-46-9/benzylamine; 57-56-7/carbamylhydrazine; EC 1.4.3.6/Amine Oxidase (Copper-Containing); EC 1.5.-/Oxidoreductases Acting on CH-NH Group Donors |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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