Document Detail


Location-dependent coronary artery diffusive and convective mass transport properties of a lipophilic drug surrogate measured using nonlinear microscopy.
MedLine Citation:
PMID:  23224981     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: Arterial wall mass transport properties dictate local distribution of biomolecules or locally delivered dugs. Knowing how these properties vary between coronary artery locations could provide insight into how therapy efficacy is altered between arterial locations.
METHODS: We introduced an indocarbocyanine drug surrogate to the lumens of left anterior descending and right coronary (LADC; RC) arteries from pigs with or without a pressure gradient. Interstitial fluorescent intensity was measured on live samples with multiphoton microscopy. We also measured binding to porcine coronary SMCs in monoculture.
RESULTS: Diffusive transport constants peaked in the middle sections of the LADC and RC arteries by 2.09 and 2.04 times, respectively, compared to the proximal and distal segments. There was no statistical difference between the average diffusivity value between LADC and RC arteries. The convection coefficients had an upward trend down each artery, with the RC being higher than the LADC by 3.89 times.
CONCLUSIONS: This study demonstrates that the convective and diffusive transport of lipophilic molecules changes between the LADC and the RC arteries as well as along their length. These results may have important implications in optimizing drug delivery for the treatment of coronary artery disease.
Authors:
Joseph T Keyes; Bruce R Simon; Jonathan P Vande Geest
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-12-07
Journal Detail:
Title:  Pharmaceutical research     Volume:  30     ISSN:  1573-904X     ISO Abbreviation:  Pharm. Res.     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-03-12     Completed Date:  2013-08-27     Revised Date:  2014-04-02    
Medline Journal Info:
Nlm Unique ID:  8406521     Medline TA:  Pharm Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1147-60     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Carbocyanines / administration & dosage,  pharmacokinetics*
Cells, Cultured
Coronary Vessels / metabolism*
Diffusion
Fluorescent Dyes / administration & dosage,  pharmacokinetics*
Image Processing, Computer-Assisted
Microscopy, Fluorescence, Multiphoton
Myocytes, Smooth Muscle / metabolism
Pressure
Swine
Grant Support
ID/Acronym/Agency:
1S10RR023737-01/RR/NCRR NIH HHS; T32 HL007955/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Carbocyanines; 0/Fluorescent Dyes
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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