Document Detail


Localizing protein-protein interactions by bimolecular fluorescence complementation in planta.
MedLine Citation:
PMID:  18586107     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The application of novel assays for basic cell research is tightly linked to the development of easy-to-use and versatile tools and protocols for implementing such technologies for a wide range of applications and model species. The bimolecular fluorescence complementation (BiFC) assay is one such novel method for which tools and protocols for its application in plant cell research are still being developed. BiFC is a powerful tool which enables not only detection, but also visualization and subcellular localization of protein-protein interactions in living cells. Here we describe the application of BiFC in plant cells while focusing on the use of our versatile set of vectors which were specifically designed to facilitate the transformation, expression and imaging of protein-protein interactions in various plant species. We discuss the considerations of using our system in various plant model systems, the use of single versus multiple expression cassettes, the application of our vectors using various transformation methods and the use of internal fluorescent markers which can assist in signal localization and easy data acquisition in living cells.
Authors:
Vitaly Citovsky; Yedidya Gafni; Tzvi Tzfira
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2008-06-27
Journal Detail:
Title:  Methods (San Diego, Calif.)     Volume:  45     ISSN:  1095-9130     ISO Abbreviation:  Methods     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-08-04     Completed Date:  2008-11-18     Revised Date:  2008-11-24    
Medline Journal Info:
Nlm Unique ID:  9426302     Medline TA:  Methods     Country:  United States    
Other Details:
Languages:  eng     Pagination:  196-206     Citation Subset:  IM    
Affiliation:
Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794-5215, USA.
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MeSH Terms
Descriptor/Qualifier:
Biological Assay / methods
Biological Markers / analysis
Fluorescent Dyes / analysis,  metabolism
Genetic Vectors
Luminescent Proteins / analysis*,  genetics,  metabolism
Microscopy, Fluorescence / methods*
Mutagenesis, Insertional
Plants / genetics,  metabolism*
Plasmids
Protein Binding
Protein Interaction Mapping / methods*
Protoplasts / metabolism
Recombinant Fusion Proteins / analysis,  metabolism,  radiation effects
Transfection
Transformation, Genetic
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Fluorescent Dyes; 0/Luminescent Proteins; 0/Recombinant Fusion Proteins

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