Document Detail


Localized proton magnetic resonance spectroscopy of lipids in adipose tissue at high spatial resolution in mice in vivo.
MedLine Citation:
PMID:  18024705     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We describe a localized proton magnetic resonance spectroscopy ((1)H-MRS) method for in vivo measurement of lipid composition in very small voxels (1.5 mm x 1.5 mm x 1.5 mm) in adipose tissue in mice. The method uses localized point-resolved spectroscopy to collect (1)H spectra from voxels in intra-abdominal white adipose tissue (WAT) and brown adipose tissue (BAT) deposits. Nonlinear least-squares fits of the spectra in the frequency domain allow for accurate calculation of the relative amount of saturated, monounsaturated, and polyunsaturated fatty acids. All spectral data are corrected for spin-spin relaxation. The data show BAT of NMRI mice to be significantly different from BAT of NMRI nu/nu mice in all aspects except for the fraction of monounsaturated fatty acids (FM); for WAT, only the FM is different. BAT and WAT of NMRI mice differ in the amount of saturated and di-unsaturated fatty acids. This method provides a potential tool for studying lipid metabolism in small animal models of disease during the initiation, progression, and manifestation of obesity-related disorders in vivo. Our results clearly demonstrate that localized (1)H-MRS of adipose tissue in vivo is possible at high spatial resolution with voxel sizes down to 3.4 ml.
Authors:
Klaus Strobel; Joerg van den Hoff; Jens Pietzsch
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Publication Detail:
Type:  Comparative Study; Journal Article     Date:  2007-11-16
Journal Detail:
Title:  Journal of lipid research     Volume:  49     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-01-17     Completed Date:  2008-04-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  473-80     Citation Subset:  IM    
Affiliation:
Institute of Radiopharmacy, Research Center Dresden-Rossendorf, Dresden, Germany. k.strobel@fzd.de
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MeSH Terms
Descriptor/Qualifier:
Adipose Tissue, Brown / chemistry*,  metabolism
Adipose Tissue, White / chemistry*,  metabolism
Animals
Female
Lipids / chemistry*
Magnetic Resonance Spectroscopy / methods*
Mice
Mice, Inbred Strains
Mice, Nude
Protons*
Chemical
Reg. No./Substance:
0/Lipids; 0/Protons

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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