Document Detail


Localized delivery of DNA to the cells by viral collagen-loaded silica colloidal crystals.
MedLine Citation:
PMID:  17824389     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Low-molecular-weight colloidal crystals with enhanced biocompatibility and ordered porous structure are used in drug-delivery systems. The objective of our study is to demonstrate the use of silica nanoscale colloid particles for localized recombinant DNA release. The colloids were coated with collagen-containing viral vector constructs of lentiviral green fluorescent protein (GFP), and solidified at 37 degrees C. The colloid-collagen-viral vector platform (CCP) was transferred to cell monolayer cultures of human lung fibroblasts. Results show specific infection of cells directly beneath the platform, as evidenced by positive GFP in their cytoplasm, while neighboring cells show no cytoplasmic GFP The infection of specific cells is probably due to the gradual release of viral particles from the collagen matrix by cell-secreted collagenase, which avoids overdosing the cells with viral particles, resulting from the cytopathic effect often seen with high-titer viral infection. Cells infected with the lentiviral-GFP or lentivirus alone, not incorporated into the colloid-collagen device, show caspase 3-associated apoptotic cell death. This suggests that colloidal crystal-coated collagen may be used as a powerful platform to deliver genes of choice to localized subgroups of specific cells of interest. This specificity in the delivery mode is beneficial for functional studies of gene-directed impact on a particular cell population of interest in a heterogeneous cell culture.
Authors:
Harshini Sarojini; Krishnakiran Medepalli; Derek A Terry; Bruce W Alphenaar; Eugenia Wang
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  BioTechniques     Volume:  43     ISSN:  0736-6205     ISO Abbreviation:  BioTechniques     Publication Date:  2007 Aug 
Date Detail:
Created Date:  2007-09-10     Completed Date:  2007-10-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8306785     Medline TA:  Biotechniques     Country:  England    
Other Details:
Languages:  eng     Pagination:  213-4, 216-8, 220-1     Citation Subset:  IM    
Affiliation:
University of Louisville School of Medicine, Louisville, KY, USA.
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MeSH Terms
Descriptor/Qualifier:
Cell Line
Colloids / chemistry
Crystallization / methods
DNA / administration & dosage*,  genetics*
Drug Carriers / chemistry*
Fibroblasts / physiology*
Humans
Lentivirus / genetics*
Silicon Dioxide / chemistry
Chemical
Reg. No./Substance:
0/Colloids; 0/Drug Carriers; 7631-86-9/Silicon Dioxide; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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