Document Detail

Localization of transglutaminase activity in type II epithelial cell cultures and elevation of enzyme activity in lungs of rats instilled with quartz.
MedLine Citation:
PMID:  2907074     Owner:  NLM     Status:  MEDLINE    
Transglutaminase activity, assessed by the incorporation of [14C]-putrescine into N-acetylated dephosphorylated beta-casein, was not detectable in sonicates of alveolar macrophages and fibroblasts but was located in all preparations of rat alveolar type II cells. Enzyme activity was induced in these cells up to 7 days in vitro but not stimulated further by the direct addition of quartz to the cultures. Transglutaminase activity in whole lung sonicates increased significantly after short-term exposure to DQ-12 quartz. An increase in the numbers of type II cells and subsequent release of activated transglutaminase, concomitant with a quartz-induced elevation in lung calcium levels and potential protein substrates, is likely to lead to an increase in protein crosslinking both in the alveolar space and interstitium.
A L Hind; C G Curtis; J Atkins; G M Powell; R J Richards
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Lung     Volume:  166     ISSN:  0341-2040     ISO Abbreviation:  Lung     Publication Date:  1988  
Date Detail:
Created Date:  1989-04-25     Completed Date:  1989-04-25     Revised Date:  2009-09-29    
Medline Journal Info:
Nlm Unique ID:  7701875     Medline TA:  Lung     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  339-46     Citation Subset:  IM    
Department of Biochemistry, University College Cardiff, Wales, United Kingdom.
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MeSH Terms
Cells, Cultured
Epithelial Cells
Epithelium / enzymology
Fibroblasts / drug effects,  enzymology
Lung / cytology*,  enzymology
Macrophages / drug effects,  enzymology
Pulmonary Alveoli / cytology,  enzymology
Quartz / pharmacology*
Silicon Dioxide / pharmacology*
Transglutaminases / metabolism*
Grant Support
//Wellcome Trust
Reg. No./Substance:
14808-60-7/Quartz; 7631-86-9/Silicon Dioxide; EC

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