Document Detail

Localization of sulfated sialic acids in the dentinal tubules during tooth formation in mice.
MedLine Citation:
PMID:  15866285     Owner:  NLM     Status:  MEDLINE    
Lectin-like properties of the major enamel protein amelogenin suggest that it binds to glycoconjugates in dentinal tubules released at the dentin-enamel junction (DEJ) during enamel formation. Therefore, a detailed mapping of glycosylation in dentinal tubules during tooth formation was undertaken using histochemistry and lectin-binding assays. The tubular content exhibited sialidase-susceptible gamma-metachromasia with Toluidine Blue (pH 2.5) and staining with Alcian Blue (pH 1.0). The presence of sulfate groups was confirmed by benzidine reactions (Bracco-Curti's and tetrazonium assays). Alpha2,3-, alpha2,6- and alpha2,8-sialidases entirely abolished staining with the benzidine reactions. The presence of sialic acids in dentinal tubules was confirmed with the Bial's reaction and sialidase-susceptible binding of Limax flavus lectin suggesting that sialic acids are the major sulfated sugars in the glycoconjguates. Immunostaining with the monoclonal antibody 5-D-4 before and after treatment with chondroitin-4- and chondroitin-6-sulfatase confirmed the presence of keratan sulfate (KS), a sialylated proteoglycan, in dentinal tubules. We suggest that sulfated sialic acids are part of the KSs. The sulfated glycoconjugates are also found in dentin and the DEJ but not in predentin suggesting that amelogenin binds to the sialoconjugate during enamel formation.
Rajeswari M H Ravindranath; Rajam M Basilrose
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S.     Date:  2005-02-24
Journal Detail:
Title:  Acta histochemica     Volume:  107     ISSN:  0065-1281     ISO Abbreviation:  Acta Histochem.     Publication Date:  2005  
Date Detail:
Created Date:  2005-05-03     Completed Date:  2005-09-22     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370320     Medline TA:  Acta Histochem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  43-56     Citation Subset:  IM    
Center for Craniofacial Molecular Biology, School of Dentistry, University of Southern California, Los Angeles, CA 90033, USA.
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MeSH Terms
Dental Enamel / chemistry,  metabolism,  ultrastructure
Dentin / chemistry*,  metabolism,  ultrastructure
Hydrogen-Ion Concentration
Keratan Sulfate / biosynthesis,  chemistry
Lectins / chemistry
Sialic Acids / analysis*,  biosynthesis
Species Specificity
Staining and Labeling
Sulfates / analysis*
Tooth / growth & development*,  metabolism,  ultrastructure
Grant Support
R01-DE-13204-05/DE/NIDCR NIH HHS
Reg. No./Substance:
0/Lectins; 0/Sialic Acids; 0/Sulfates; 9056-36-4/Keratan Sulfate

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