| Localization of satellite DNAs in the chromosomes of the guinea pig. | |
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MedLine Citation:
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PMID: 391515 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The in situ hybridization method has been used to investigate the localization of each of the three satellite DNAs present in the genome of the guinea pig. Purified fractions of the satellite DNAs were utilized as templates for synthesis of 3H-labeled complementary RNA (cRNA) by E. coli RNA polymerase, then each cRNA was hybridized to metaphase spreads of embryonic guinea pig cells. The cRNAs of all three satellite DNAs hybridized predominantly to the centromeric region of the chromosomes. The cRNAs of satellite DNAs II and III hybridized to all chromosomes except the Y chromosome. The cRNA of satellite DNA I did not hybridize to the Y chromosome nor to two pairs of small acrocentric chromosomes. Satellite II cRNA hybridized to the telomeric region of chromosomes 3 and 4. |
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Authors:
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N Duhamel-Maestracci; R Simard; K Harbers; J H Spencer |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Chromosoma Volume: 75 ISSN: 0009-5915 ISO Abbreviation: Chromosoma Publication Date: 1979 Oct |
Date Detail:
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Created Date: 1980-03-24 Completed Date: 1980-03-24 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 2985138R Medline TA: Chromosoma Country: GERMANY, WEST |
Other Details:
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Languages: eng Pagination: 63-74 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chromosomes / ultrastructure* DNA, Satellite* / genetics Escherichia coli / genetics Guinea Pigs / genetics* Nucleic Acid Hybridization RNA, Bacterial / genetics Transcription, Genetic |
| Chemical | |
Reg. No./Substance:
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0/DNA, Satellite; 0/RNA, Bacterial |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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