| Localization of the renin-angiotensin system in the bovine ovary: cyclic variation of the angiotensin II receptor expression. | |
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MedLine Citation:
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PMID: 11717127 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Angiotensin (Ang) II may modulate reproductive function in the bovine ovary. Therefore, expression and localization of a local ovarian renin-angiotensin system (RAS) were investigated by elucidating the influence of the estrus cycle, pregnancy, and the presence of follicular cysts. Receptor analysis and autoradiography were used to characterize and localize Ang II receptors. Cyclic variations in the density of ovarian Ang II receptors were found with a higher value in estrus than in diestrus. The density in ovaries with follicular cysts was in the same order of magnitude as in estrus. The Ang II receptor type 2 (AT(2)) dominated in all three groups. Autoradiography showed that the majority of antral follicles and follicular cysts had intense AT(2) receptor binding in the theca externa. Binding was less intense in the theca interna, whereas there was no binding in the granulosa layer. In the corpora lutea, the AT(2) receptor was dominant in the capsule and in connective tissue infoldings, whereas no binding was observed in the luteal tissue. The type 1 Ang II receptor (AT(1)) was dominant in the stroma and showed no cyclic changes. Angiotensin-converting enzyme (ACE) activity was detected in all aspirated follicular fluids and homogenates of ovarian tissue. Autoradiography showed that most of the ACE was localized on endothelial cells. Renin immunoreactivity was found in granulosa and thecal cells of antral follicles and in luteal cells. Furthermore, solitary cells in the stroma, presumably macrophages, displayed intense staining. Our finding of cyclic changes support the concept of an active and regulated RAS in the bovine ovary. |
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Authors:
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K H Schauser; A H Nielsen; H Winther; V Dantzer; K Poulsen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biology of reproduction Volume: 65 ISSN: 0006-3363 ISO Abbreviation: Biol. Reprod. Publication Date: 2001 Dec |
Date Detail:
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Created Date: 2001-11-21 Completed Date: 2002-01-31 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0207224 Medline TA: Biol Reprod Country: United States |
Other Details:
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Languages: eng Pagination: 1672-80 Citation Subset: IM |
Affiliation:
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Department of Anatomy and Physiology, The Royal Veterinary and Agricultural University, DK-1870 Frederiksberg C, Denmark. ks@kvl.dk |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Autoradiography Cattle Cell Membrane / chemistry Corpus Luteum / chemistry Estrous Cycle / physiology Female Follicular Fluid / enzymology Granulosa Cells / chemistry Ovary / chemistry*, metabolism Peptidyl-Dipeptidase A / analysis Pregnancy Receptor, Angiotensin, Type 2 Receptors, Angiotensin / analysis*, metabolism Renin / analysis Renin-Angiotensin System* Stromal Cells / chemistry Theca Cells / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Receptor, Angiotensin, Type 2; 0/Receptors, Angiotensin; EC 3.4.15.1/Peptidyl-Dipeptidase A; EC 3.4.23.15/Renin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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