Document Detail


Localization and regulation of the putative membrane fatty-acid transporter (FAT) in the small intestine. Comparison with fatty acid-binding proteins (FABP).
MedLine Citation:
PMID:  8681947     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The expression of the putative membrane fatty-acid transporter (FAT) was investigated in the small intestine. The FAT mRNA level was higher in the jejunum than in the duodenum and was lower in the ileum, as observed for cytosolic fatty-acid-binding proteins (FABP) expressed in this tissue. No FAT transcript was found in the stomach or colon. FAT mRNA was constitutively expressed in the epithelial cells located in the upper two thirds of villi, while it was undetectable in the crypt cells and submucosal cells. In jejunal mucosa, immunochemical studies showed that FAT protein was limited to the brush border of enterocytes. No fluorescence was found in the goblet cells. To determine whether FAT responded to changes in fat intake, as reported for FABP, the effect of two high-fat diets, which essentially contained either medium-chain fatty acids or long-chain fatty acids (sunflower-oil diet), was investigated. The sunflower-oil diet greatly increased FAT mRNA abundance throughout the small intestine. In contrast, a weak effect of medium-chain fatty acids was observed only in the jejunum. As found for FABP expression, treatment with the hypolipidemic drug bezafibrate affected FAT expression. These data demonstrate that FAT and FABP are co-expressed in enterocytes, as has been shown in adipocytes, myocytes and mammary cells. The data suggest that these membrane and cytosolic proteins might have complementary functions during dietary-fat absorption.
Authors:
H Poirier; P Degrace; I Niot; A Bernard; P Besnard
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of biochemistry / FEBS     Volume:  238     ISSN:  0014-2956     ISO Abbreviation:  Eur. J. Biochem.     Publication Date:  1996 Jun 
Date Detail:
Created Date:  1996-08-22     Completed Date:  1996-08-22     Revised Date:  2007-07-23    
Medline Journal Info:
Nlm Unique ID:  0107600     Medline TA:  Eur J Biochem     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  368-73     Citation Subset:  IM    
Affiliation:
Laboratoire de Physiologie de la Nutrition, Ecole Nationale Supérieure de Biologie Appliquée a la Nutrition et à l'Alimentation, Université de Bourgogne, Dijon, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antilipemic Agents / pharmacology
Bezafibrate / pharmacology
Biological Transport
Blotting, Northern
Blotting, Western
Carrier Proteins / analysis,  metabolism*
Dietary Fats / administration & dosage
Fatty Acid-Binding Proteins
Fatty Acids / metabolism*
Gene Expression Regulation / genetics
Immunohistochemistry
Intestinal Mucosa / chemistry,  metabolism*
Intestine, Small / chemistry,  metabolism*
Male
Membrane Proteins / analysis,  metabolism*
Myelin P2 Protein / metabolism*
Neoplasm Proteins*
Nerve Tissue Proteins*
RNA, Messenger / analysis,  metabolism
Rats
Rats, Wistar
Chemical
Reg. No./Substance:
0/Antilipemic Agents; 0/Carrier Proteins; 0/Dietary Fats; 0/Fabp7 protein, rat; 0/Fatty Acid-Binding Proteins; 0/Fatty Acids; 0/Membrane Proteins; 0/Myelin P2 Protein; 0/Neoplasm Proteins; 0/Nerve Tissue Proteins; 0/RNA, Messenger; 41859-67-0/Bezafibrate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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