Document Detail

Localization of radiation-induced chromosomal breakpoints along human chromosome 1 using a combination of G-banding and FISH.
MedLine Citation:
PMID:  10866289     Owner:  NLM     Status:  MEDLINE    
PURPOSE: To determine the exact location of radiation-induced chromosomal breakpoints along the euchromatic or heterochromatic regions: G-light and G-dark bands, respectively. MATERIALS AND METHODS: The distribution of radiation-induced chromosomal breakpoints was scored in human lymphocytes irradiated in vitro with 3 Gy of gamma-radiation. Image analysis was applied to combine G-banded and FISH-painted images of the human chromosome 1. RESULTS: A total of 195 chromosomal breakpoints in 176 cells with structural chromosomal aberrations was used for the present analysis. Radiation-induced breakpoints were found to be distributed randomly with respect to the p or q arms of chromosome 1 and specific band or band length, but more breakpoints were mapped to G-light than to G-dark bands, the difference being statistically significant. CONCLUSIONS: The results can well be interpreted in terms of concepts of existing models of nuclear architecture, chromatin structure and transcriptional activities of the chromatin, which can influence the induction of primary chromosomal aberrations by gamma-rays. Differential repair of randomly produced primary aberrations may also explain the non-random distribution of radiation-induced breakpoints.
A Kiuru; C Lindholm; A Auvinen; S Salomaa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of radiation biology     Volume:  76     ISSN:  0955-3002     ISO Abbreviation:  Int. J. Radiat. Biol.     Publication Date:  2000 May 
Date Detail:
Created Date:  2000-07-13     Completed Date:  2000-07-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8809243     Medline TA:  Int J Radiat Biol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  667-72     Citation Subset:  IM; S    
Research and Environmental Surveillance, Radiation and Nuclear Safety Authority, Helsinki, Finland.
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MeSH Terms
Chromosome Aberrations*
Chromosome Banding
Chromosome Breakage
Chromosomes / genetics,  radiation effects*
Chromosomes, Human, Pair 1 / genetics*,  radiation effects*
DNA Damage / radiation effects
Gamma Rays
In Situ Hybridization, Fluorescence
Lymphocytes / radiation effects
Metaphase / radiation effects

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