Document Detail


Localization and quantification of 5-hydroxytryptophan and serotonin in the central nervous systems of Tritonia and Aplysia.
MedLine Citation:
PMID:  11477599     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Serotonin (5-hydroxytryptamine, 5-HT) plays a central role in several behaviors in marine molluscs and other species. In an effort to better understand the regulation of 5-HT synthesis, we used high performance liquid chromatography (HPLC) with electrochemical detection and immunohistochemistry to measure and map the distribution of the immediate precursor of 5-HT, 5-hydroxytryptophan (5-HTP), in two model opisthobranch molluscs, the nudibranch Tritonia diomedea and the anaspid Aplysia californica. HPLC measurements showed that 5-HTP is present at approximately the same level as the 5-HT metabolite, 5-hydroxyindolacetic acid (5-HIAA) but is more than 100 times lower in concentration than either 5-HT or dopamine in the same tissue. Specific 5-HTP immunoreactivity was colocalized with serotonin in both species. The overall intensity of 5-HTP immunoreactivity in individual ganglia agreed with HPLC measurements for those ganglia. The intensity of 5-HTP immunolabeling varied between cell types and was correlated with the intensity of 5-HT immunolabeling. In particular, differences in staining intensity were consistently seen among the three dorsal swim interneurons of the Tritonia swim central pattern generator circuit. Some nonserotonergic neurons also displayed low levels of 5-HTP immunolabeling that were above background levels. Together, these results support the notion that production of 5-HTP is a rate-limiting step in serotonin synthesis and suggest that there may be additional regulation that allows 5-HTP to accumulate to varying levels.
Authors:
D J Fickbohm; C P Lynn-Bullock; N Spitzer; H K Caldwell; P S Katz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of comparative neurology     Volume:  437     ISSN:  0021-9967     ISO Abbreviation:  J. Comp. Neurol.     Publication Date:  2001 Aug 
Date Detail:
Created Date:  2001-07-30     Completed Date:  2001-09-13     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0406041     Medline TA:  J Comp Neurol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  91-105     Citation Subset:  IM    
Copyright Information:
Copyright 2001 Wiley-Liss, Inc.
Affiliation:
Center for Behavioral Neuroscience, Department of Biology, Georgia State University, Atlanta, GA 30303, USA.
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MeSH Terms
Descriptor/Qualifier:
5-Hydroxytryptophan / analysis*,  immunology
Animals
Antibodies
Aplysia / chemistry*
Central Nervous System / chemistry,  cytology
Chromatography, High Pressure Liquid
Dopamine / analysis
Ganglia, Invertebrate / chemistry,  cytology
Hydroxyindoleacetic Acid / analysis,  immunology
Immunohistochemistry
Microscopy, Confocal
Neurons / chemistry
Serotonin / analysis*,  immunology
Grant Support
ID/Acronym/Agency:
NS35371/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies; 50-67-9/Serotonin; 54-16-0/Hydroxyindoleacetic Acid; 56-69-9/5-Hydroxytryptophan

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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