Document Detail


Localization of preproenkephalin A mRNA in the neonatal rat retina.
MedLine Citation:
PMID:  1786557     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An opioid growth factor, [Met5]-enkephalin, is known to regulate developmental events in the neonatal rat retina. This growth factor interacts with the zeta (zeta) opioid receptor to modulate retinal ontogeny. Both peptide and receptor are present in developing retina, but not in adult retina. We have used in situ hybridization histochemistry to identify and localize preproenkephalin A mRNA in the neonatal rat retina. Preproenkephalin mRNA was localized to the ganglion cell layer, with some radiolabeling found in the neuroblast layer. This result indicates that 1) the mRNA to preproenkephalin A is present during the critical stage of development in the neonatal retina that coincides with the presence of the growth-regulating peptide, [Met5]-enkephalin, and 2) that the source of the opioid growth factor controlling the production of retinal cells appears to be autocrine (i.e., retinal neuroblasts) and paracrine (i.e., ganglion cells) in nature.
Authors:
T Isayama; I S Zagon
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Brain research bulletin     Volume:  27     ISSN:  0361-9230     ISO Abbreviation:  Brain Res. Bull.     Publication Date:  1991 Dec 
Date Detail:
Created Date:  1992-03-24     Completed Date:  1992-03-24     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7605818     Medline TA:  Brain Res Bull     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  805-8     Citation Subset:  IM    
Affiliation:
Department of Neuroscience and Anatomy, Milton S. Hershey Medical Center, Pennsylvania State University, Hershey 17033.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn
Autoradiography
Enkephalins / genetics*
Female
Histocytochemistry
Male
Nucleic Acid Hybridization
Oligonucleotide Probes
Protein Precursors / genetics*
RNA, Messenger / metabolism*
Rats
Rats, Inbred Strains
Retina / metabolism*
Tissue Distribution
Grant Support
ID/Acronym/Agency:
NS-20500/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Enkephalins; 0/Oligonucleotide Probes; 0/Protein Precursors; 0/RNA, Messenger; 93443-35-7/preproenkephalin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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