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Localization of phosphatidylinositol phosphate kinase IIgamma in kidney to a membrane trafficking compartment within specialized cells of the nephron.
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PMID:  18753295     Owner:  NLM     Status:  MEDLINE    
PIP4Ks (type II phosphatidylinositol 4-phosphate kinases) are phosphatidylinositol 5-phosphate (PtdIns5P) 4-kinases, believed primarily to regulate cellular PtdIns5P levels. In this study, we investigated the expression, localization, and associated biological activity of the least-studied PIP4K isoform, PIP4Kgamma. Quantitative RT-PCR and in situ hybridization revealed that compared with PIP4Kalpha and PIP4Kbeta, PIP4Kgamma is expressed at exceptionally high levels in the kidney, especially the cortex and outer medulla. A specific antibody was raised to PIP4Kgamma, and immunohistochemistry with this and with antibodies to specific kidney cell markers showed a restricted expression, primarily distributed in epithelial cells in the thick ascending limb and in the intercalated cells of the collecting duct. In these cells, PIP4Kgamma had a vesicular appearance, and transfection of kidney cell lines revealed a partial Golgi localization (primarily the matrix of the cis-Golgi) with an additional presence in an unidentified vesicular compartment. In contrast to PIP4Kalpha, bacterially expressed recombinant PIP4Kgamma was completely inactive but did have the ability to associate with active PIP4Kalpha in vitro. Overall our data suggest that PIP4Kgamma may have a function in the regulation of vesicular transport in specialized kidney epithelial cells.
Jonathan H Clarke; Piers C Emson; Robin F Irvine
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-08-27
Journal Detail:
Title:  American journal of physiology. Renal physiology     Volume:  295     ISSN:  1931-857X     ISO Abbreviation:  Am. J. Physiol. Renal Physiol.     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2008-11-06     Completed Date:  2009-02-09     Revised Date:  2013-06-05    
Medline Journal Info:
Nlm Unique ID:  100901990     Medline TA:  Am J Physiol Renal Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  F1422-30     Citation Subset:  IM    
Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge CB2 1PD, UK.
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MeSH Terms
Aquaporin 1 / genetics,  metabolism
Autoantigens / genetics,  metabolism
Blotting, Western
COS Cells
Cell Line
Cercopithecus aethiops
Gene Expression
Golgi Apparatus / enzymology,  metabolism
HeLa Cells
In Situ Hybridization
Isoenzymes / genetics,  metabolism
Kidney / cytology,  enzymology*,  metabolism
Kidney Cortex / cytology,  enzymology,  metabolism
Kidney Medulla / cytology,  enzymology,  metabolism
Loop of Henle / cytology,  enzymology,  metabolism
Membrane Proteins / genetics,  metabolism
Mice, Inbred Strains
Mucoproteins / genetics,  metabolism
Nephrons / cytology,  enzymology*,  metabolism
Phosphotransferases (Alcohol Group Acceptor) / chemistry,  genetics,  metabolism*
Reverse Transcriptase Polymerase Chain Reaction
Transport Vesicles / enzymology*,  metabolism
Grant Support
WT063581//Wellcome Trust; //Biotechnology and Biological Sciences Research Council
Reg. No./Substance:
0/Aqp1 protein, mouse; 0/Autoantigens; 0/Golgin subfamily A member 2; 0/Isoenzymes; 0/Membrane Proteins; 0/Mucoproteins; 0/UMOD protein, human; 0/Umod protein, mouse; 0/Uromodulin; 146410-94-8/Aquaporin 1; EC 2.7.1.-/Phosphotransferases (Alcohol Group Acceptor); EC phosphate 4-kinase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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Journal Information
Journal ID (nlm-ta): Am J Physiol Renal Physiol
Journal ID (publisher-id): ajprenal
ISSN: 0363-6127
ISSN: 1522-1466
Publisher: American Physiological Society
Article Information
Copyright ? 2008, American Physiological Society
open-access: This document may be redistributed and reused, subject to
Received Day: 15 Month: 5 Year: 2008
Accepted Day: 24 Month: 8 Year: 2008
Print publication date: Month: 11 Year: 2008
Electronic publication date: Day: 27 Month: 8 Year: 2008
pmc-release publication date: Day: 1 Month: 11 Year: 2008
Volume: 295 Issue: 5
First Page: F1422 Last Page: F1430
ID: 2584910
Publisher Id: F-90310-2008
DOI: 10.1152/ajprenal.90310.2008
PubMed Id: 18753295

Localization of phosphatidylinositol phosphate kinase II? in kidney to a membrane trafficking compartment within specialized cells of the nephron
Jonathan H. Clarke1
Piers C. Emson2
Robin F. Irvine1
1Department of Pharmacology, University of Cambridge and 2Laboratory of Molecular Neuroscience, Babraham Institute, Cambridge, United Kingdom
Address for reprint requests and other correspondence: J. H. Clarke, Dept. of Pharmacology, Univ. of Cambridge, Tennis Court Road, Cambridge CB2 1PD, UK (e-mail:

POLYPHOSPHOINOSITIDES are quantitatively minor lipid components of cells and are increasingly being recognized as making diverse and important contributions to many aspects of cell physiology, for example, in ion channel regulation, membrane trafficking, cell proliferation, and cytoskeletal rearrangement (for reviews see Refs. 10, 18, 32, 40, 46). Phosphatidylinositol 5-phosphate (PtdIns5P) is the most recent polyphosphoinositide to be found, first identified as the primary substrate for the type II PtdInsP kinases (33). Here these enzymes are referred to as PIP4Ks (consistent with their substrate specificity as PtdIns5P 4-kinases), although it should be noted that gene nomenclature still refers to them as PIP5K2s. As a minor component of cell polyphosphoinositides, it is suggested that PtdIns5P either provides a specific lipid pool for a (minor) localized production of PtdIns(4,5)P2 or that it is a signaling molecule in its own right. Suggested functions include nuclear responses (9, 13), insulin signaling, and protein kinase regulation (5, 22, 31, 38) and phosphatase activation (39).

PtdIns5P can be synthesized from PtdIns by a PtdIns 5-kinase (37) or by dephosphorylation from PtdIns(3,5)P2 or PtdIns(4,5)P2 (28, 41?43). The main route of PtdIns5P metabolism is via PIP4Ks, which are represented in vertebrates by three characterized isoforms, ?, ?, and ?. PIP4K? is found predominantly in the cytosol and can be recruited to the plasma membrane (15, 45). PIP4K? is localized to the nucleus (3, 7, 36), where there is evidence that it regulates PtdIns5P levels (21), probably in concert with type I PtdIns(4,5)P2 4-phosphatase (47), although it has also been seen to associate with the cytosolic TNF receptor (6). As cellular PtdIns5P levels are modified in response to stress (21, 47), to bacterial infection (28), to receptor-mediated signaling (26, 45), and during cell cycle progression (8), the ability of the PIP4Ks to localize to specific compartments, or to shuttle between them, may be intrinsic to their function.

The third isoform, PIP4K? (19), has not yet been associated with a cellular function, either related to production of PtdIns(4,5)P2 or to the attenuation of PtdIns5P. In the present study, we have extensively characterized the tissue distribution of all of the PIP4K isoforms and found an exceptionally high level of PIP4K? expression in the kidney. We have further investigated the localization within this organ of PIP4K?, using a specific antibody to this isoform, and found that it is remarkably confined to specific cell populations, where it is localized to vesicular structures. Transfection experiments with PIP4K? suggest that these may be derived from the Golgi apparatus. Our results suggest that PIP4K? may be involved in vesicle trafficking in specific kidney cells and that this would imply a specialized function for this enzyme.

PIP4K cloning.

PIP5K2 genes were amplified from a whole human brain marathon-ready cDNA library (Clontech Laboratories, Mountain View, CA) using gene-specific primers (PIP5K2A forward: 5?-ATGGCGACCCCCGGCAACCTAGGGTC-3? and reverse: 5?-TTACGTCAAGATGTGGCCAATAAAGTC-3?; PIP5K2B forward: 5?-ATGTCGTCCAACTGCACCAGCACCAC-3? and reverse: 5?-CTACGTCAGGATGTTGGACATAAAC-3?; and PIP5K2C forward: 5?-ATGGCGTCCTCCTCGGTCCCACCAG-3? and reverse: 5?-TTAGGCAAAGATGTTGGTAATAAAATC-3?). PCR products were cloned, via incorporated HindIII and BamHI restriction sites, into appropriate plasmid vectors. Recombinant protein expressed from Escherichia coli harboring PIP5K2s in pET-32a (Novagen, Madison, WI) was purified using TALON metal affinity resin (Clontech) and cleaved with enterokinase (New England Biolabs, Ipswich, MA). Endotoxin-free plasmid from bacterial clones harboring PIP5K2s in pEGFP-C1 (Clontech) was prepared (DNA extraction kit; Qiagen, Huntsville, AL). All constructs were confirmed by sequencing.

Cell culture and transfection.

HeLa, HEK 293, COS-7, and NRK cells were maintained in DMEM (GIBCO, Paisley, UK) supplemented with 10% fetal bovine serum, 50 U/ml penicillin, and 50 ?g/ml streptomycin. Cells were transiently transfected with plasmid constructs for 24 h with TransFectin reagent (Bio-Rad, Hercules, CA), using the manufacturer's protocol. Cell lysates for immunoprecipitation were made by suspending cells in cold lysis buffer (PBS with 1% Triton X-100, 5 mM EDTA, 5 mM EGTA, and 100 ?l/ml Sigma P8340 protease inhibitor cocktail) and centrifuging at 10,000 g for 10 min at 4?C.

Tissue sample preparation.

Protein and mRNA samples were prepared from adult mouse tissues obtained post mortem and immediately frozen on dry ice after collection. Tissues (50 mg) for mRNA extraction were pulsed in lysing matrix D tubes (Qbiogene) with 1 ml Tri-Reagent (Sigma-Aldrich, Poole, UK) on a Hybaid Ribolyser. Samples were purified (RNeasy kit; Qiagen, and Turbo DNase; Ambion, Austin, TX), and cDNA libraries were constructed by RT-PCR (Sprint Powerscript kit; Clontech). Protein lysates were prepared in RIPA buffer (150 mM sodium chloride, 50 mM Tris pH 7.4, 1 mM EDTA, 1% Triton X-100, 1% deoxycholic acid, and 0.1% SDS) with 10 mM tetrasodium pyrophosphate, 10 mM sodium fluoride, 17.5 mM ?-glycerophosphate, and 100 ?l/ml Sigma P8340 protease inhibitor cocktail, using 10?15 strokes in a 5 ml dounce homogenizer. Cell debris was cleared by centrifugation at 2,900 g for 20 min at 4?C, and lysates were clarified by further centrifugation at 29,000 g for 45 min at 4?C. Kidney tissue was dissected under a binocular microscope to produce cortical and medullary samples. Tissues for immunochemistry from perfused mice were fixed in 4% paraformaldehyde, sectioned on a Leica cryostat at ?20?C, mounted on slides, and stored at ?80?C.

Quantitative PCR.

Singleplex quantitative PCR was carried out using specific primers for each PIP5K2 gene, designed to the 3?-untranslated region (PIP5K2A forward: 5?-AAGAGTCTGATGCCAAGAACCTGT-3? and reverse: 5?-TGCAGTGCAACTTAAGGATGGTAA-3?; PIP5K2B forward: 5?-CATCCTCACAGAAGAACATGGC-3? and reverse: 5?-CCTGGTCATTCACCGTCTCA-3?; and PIP5K2C forward: 5?-CATCTTCCACTGCTAATGTGTCTCC-5? and reverse: 5?-TTGAGTTATGGCTCTGACTCCTCTCT-3?). Three cDNA libraries were constructed for each tissue tested and analyzed in triplicate. PCR amplification was performed in a 96-well plate using SYBR Green I master mix (Applied Biosystems, Warrington, UK), following the manufacturer's protocol. Reactions were heated (50?C for 2 min, 95?C for 10 min) and cycled 40 times (95?C for 15 sec, 60?C for 1 min) on an ABI Prism 7700 sequence detection system (Applied Biosystems). Dissociation curves for each primer set indicated a single product, and no-template controls were negative after 40 cycles. Reactions using RNA as template were negative, showing that the preparations were free from genomic DNA contamination. Primer concentrations were chosen to give threshold cycle (CT) values of 20?25. Validation experiments showed equivalent relative amplification efficiencies between each PIP5K2 gene and a primer set for mouse ?-actin (forward: 5?-GACGATATCGCTGCGCTGGT-3? and reverse: 5?-CCACGATGGAGGGGAATA-3?), and ?CT values were analyzed using the comparative CT method (23).

In situ hybridization.

Two oligonucleotide probes were designed to the PIP5K2C sequence, one in the 3?-untranslated region (5?-GACTGGGTGGATTGAGTTATGGCTCTGACTCCTCT-3?) and one in the coding sequence (5?-ATAGGAGATAAGGAAACGGCCATCACTGCCTTCAG-3?), which only identified PIP5K2C when BLAT searched against the European Molecular Biology Laboratory mouse database. Probes were 3?-tail-labeled with [35S]dATP (NEN, Hounslow, UK), hybridized with 20-?m mouse tissue sections and autoradiographed for 5 wk, as described previously (12). Slides of interest were dipped in autoradiographic emulsion and after development (12 wk) were counterstained with hematoxylin and eosin, and images were captured using an Axioskop II light microscope.

Antibody analysis and Western blotting.

A peptide (amino acids 333?352), unique to the variable region of the mouse PIP4K? sequence, was used to raise a custom polyclonal antibody (NeoMPS, San Diego, CA), which was subsequently purified from rabbit serum by affinity matrix chromatography. Surface plasmon resonance analysis was carried out on a Biacore 3000 instrument (GE Healthcare Life Sciences, Bucks, UK) using two optical biosensor chips: carboxymethylated dextran preimmobilized with nitrilotriacetic acid (NTA) or streptavidin (SA). Purified PIP4K recombinant protein and control 6xHis tag protein were adsorbed to the NTA chip at concentrations of 1?9 ng/mm2. Anti-PIP4K? antibody was passed over the chip at 10 ?g/ml to detect specific interaction, and data were normalized to the nonspecific control. Biotinylated anti-PIP4K? and control antibodies were bound to the SA chip, to which PIP4K? was then applied at saturating levels, to show specific antibody-binding interaction.

Protein samples (50 ?g) were resolved on 10% polyacrylamide gels, and Western blots were carried out as described previously (16), using anti-PIP4K? at 0.5 ?g/ml. Anti-PIP4K? was neutralized by saturating with excess of antigenic peptide for 30 min. Other antibodies used in this study were diluted to the manufacturers recommendations: goat polyclonal antibodies to aquaporin 1 (AQP1), aquaporin 2 (AQP2) and Tamm-Horsfall protein, and polyclonal rabbit antibodies to lgp110 (Santa Cruz Biotechnology, Santa Cruz, CA); rabbit polyclonal antibodies to TGN38 were a gift from G. Banting; mouse Mabs to EEA1, BiP, and GM130, and rabbit polyclonal antibodies to p115 (BD Transduction Laboratories, Oxford, UK); rabbit polyclonal antibodies to calreticulin (Calbiochem, La Jolla, CA); mouse Mabs to tubulin (Sigma-Aldrich); and goat polyclonal antibodies to golgin160 and rabbit polyclonal antibodies to catalase, p58K, mannose 6-phosphate receptor, and mannosidase II (Abcam, Cambridge, UK). Alexa Fluor 568 phalloidin and TO-PRO-3 iodide were used to directly stain actin and DNA (Molecular Probes, Paisley, UK). Horseradish peroxidase-conjugated secondary antibodies and SuperSignal West Dura substrate were used for Western blotting (Pierce Protein Research Products, Rockford, IL), and Alexa dye-conjugated secondary antibodies were used for fluorescence microscopy (Molecular Probes). Immunoprecipitation with anti-PIP4K? rat Mabs (16) and protein G-Sepharose (GE Healthcare) was carried out for 16 h at 4?C. Beads were washed in cold PBS and used directly for Western blotting or kinase assay.

Immunocytochemistry and immunohistochemistry.

Mammalian cells expressing green fluorescent protein-tagged constructs were fixed in 4% paraformaldehyde for 30 min on ice. Cells were permeabilized with 0.1% Triton X-100 in PBS for 10 min and blocked with 4% fish skin gelatin (Sigma-Aldrich) in PBS for 30 min and then incubated for 60 min with primary and secondary antibodies (2% gelatin in PBS) with PBS washes between and after incubation. Slides were mounted using ProLong Gold antifade reagent (Molecular Probes), and images were taken on a Leica TCS SP5 laser scanning confocal microscope running LAS AF software (Leica Microsystems, Wetzlar, Germany). Golgi dissociation was achieved by treatment with Brefeldin A (10 ?g/ml in DMEM) for 30 min at 37?C.

Sagittal and horizontal tissue sections for fluorescent labeling were pretreated to reduce autofluorescence (20 min in 1 mg/ml sodium borohydride), blocked for 2 h (1% fish skin gelatin, 0.3% Triton X-100 in PBS), exposed to primary antibody (24 h at 4?C), and fluorophore-labeled secondary antibody (4 h) before being mounted as described previously. Confocal images were spectrally separated by LAS AF software using reference spectra obtained from unstained tissue and cells overexpressing green fluorescent protein. Tissue sections for chemical staining were pretreated for 20 min (20% methanol and 5% hydrogen peroxide), blocked, and incubated with primary antibody as described, and then they were incubated with biotinylated anti-rabbit IgG antibodies before visualization using Vectastain ABC and DAB substrate kits (Vector Laboratories, Burlingame, CA). Slides were treated with Histoclear and mounted with DPX reagent (Sigma-Aldrich) before light microscopy.

Lipid kinase assay.

PIP4K assays were carried out as described previously (16) with slight adaptation. Substrate lipid (6 ?M PtdIns5P) was dried under vacuum, and micelles were made by sonication in kinase buffer (50 mM Tris pH 7.4, 10 mM MgCl2, 80 mM KCl, and 2 mM EGTA). Recombinant PIP4K was added to the reaction mixture with 10?Ci [?-32P]ATP for 90 min at 30?C. Lipids were extracted and separated by silica-gel thin layer chromatography (16), and results were obtained by autoradiography.

PIP5K2 isoforms have different expression profiles.

Expression studies using RT-PCR have previously suggested that levels of PIP5K2 transcription are high in different tissues (1, 19). Using a series of isoform-specific PCR primer pairs for PIP5K2A, PIP5K2B, and PIP5K2C, we quantitatively assessed the expressed levels of these transcripts in mRNA isolated from a range of mouse tissues (Fig. 1). Automated quantitative PCR of synthesized cDNA libraries was completed using three biological replicates for each tissue. Pooled data were analyzed by the Livak method (23), normalizing PIP5K2 to the housekeeping gene ?-actin, and values were calculated as the relative fold increase above the lowest observed tissue expression (Fig. 1A). All three isoforms were expressed at higher levels in the brain, with PIP5K2A expression increased in spleen and PIP5K2B in muscle. PIP5K2C was especially high in kidney, as originally reported by Itoh et al. (19). Tissue analysis by in situ hybridization with a range of PIP5K2C probes confirmed that transcription of this isoform was upregulated in brain and kidney, compared with a control tissue, and that the expression was localized to discrete regions of these organs (Fig. 1B). Silver grain labeling of PIP5K2C mRNA in the kidney suggested that expression was confined to segments of the nephron within the cortical and medullary regions and was not present in the kidney vasculature (Fig. 1C).

Endogenous PIP4K? is differentially expressed in mouse tissues.

A polyclonal peptide antibody to the variable region of PIP4K? was raised and purified. Due to the similarity of the sequence of the PIP4K isoforms at the protein level, the specificity of the antibody for PIP4K? was determined (Fig. 2). The anti-PIP4K? antibody has no cross-reactivity with PIP4K? or PIP4K? by Western blot (Fig. 2A) or by immunocytochemistry (Fig. 2B). Purified recombinant PIP4Ks were bound to an NTA sensor chip by 6xHis tag, and surface plasmon resonance analysis of the binding of anti-PIP4K? antibody indicated specificity for PIP4K? compared with PIP4K? and PIP4K? (Fig. 2C). This was confirmed by binding biotinylated anti-PIP4K? antibody to an SA sensor chip and detecting specific binding to PIP4K? (data not shown). Direct Western blotting of a bank of tissue lysates confirmed the presence of significant endogenous levels of PIP4K? in brain, kidney, ovary, and testis (Fig. 3A, i). Other tissues had little or no detectable levels of PIP4K?, assuming representative expression based on equivalent loading of total lysate protein. Endogenous PIP4K? was detected as two bands of very similar molecular mass of ?47 kDa, the larger band presumably representing the phosphorylated form of mature PIP4K? (19). Crude kidney fractionation and subsequent Western blotting of protein lysates indicated that PIP4K? was present throughout this organ, but comparatively higher levels were seen in the medulla (Fig. 3B, i). Nonspecific bands were visualized using neutralized antibody as control, and lower molecular mass immunoreactive bands were presumed to be products of proteolytic cleavage (Fig. 3, ii). Interestingly, PIP4K? in heart ran predominantly as a 40-kDa band, suggesting that processing of this isoform may be occurring (Fig. 3A).

PIP4K? expression in kidney is localized to specific cells.

Identification of PIP4K? at a cellular level was shown to be specific by the use of peptide-saturated primary antibody controls, which demonstrated an absence of signal due to nonspecific binding of primary or secondary antibodies in tissue immunohistochemistry. Spectral separation was also utilized to remove significant autofluorescence from the kidney tissue in immunofluorescence experiments (Fig. 4A). Analysis of whole kidney sections confirmed that PIP4K? was expressed throughout this organ (data not shown). Detailed examination of representative regions of the kidney at high resolution indicated that the positive signal was seen to be concentrated in the outer medulla (Fig. 4B), confirming the results obtained for transcript expression (Fig. 1B), with much less signal being observed in the cortical labyrinth. This signal was restricted to whole regions of specific tubules, which could be visualized using sequential confocal imaging at 2-?m intervals throughout a 20-?m cryosectioned tissue slice (data not shown). The PIP4K?-expressing tubules were present in both the cortical medullary rays and the inner and outer stripe of the outer medulla but were absent from the tubules of the inner medulla (Fig. 4B). However, it was possible to observe single PIP4K?-positive cells within tubules in the cortex and inner medulla (Fig. 4B).

Costaining sections with selective markers for different nephron regions allowed accurate determination of the tubules and cells that expressed PIP4K? (Fig. 5). The absence of PIP4K? in cortical tubules with a significant brush-border lumen lining (Fig. 5, A and B) or coincident with the water channel AQP1 (Fig. 5C), a marker for the proximal convoluted tubule and thin descending limb of the loop of Henle (29), suggested that expression was restricted to the distal part of the nephron. PIP4K?-positive tubules also expressed Tamm-Horsfall protein in the outer medulla (Fig. 5, D?F), which suggested that PIP4K? is localized to cells in the thick ascending limb (TAL) of the loop of Henle (2). The presence of PIP4K?-positive tubules in the cortex and outer medulla (Fig. 5, G and H), but not in the inner medulla (Fig. 5I), and distinct from tubules expressing the collecting duct marker AQP2 (24), confirmed that PIP4K? was mainly expressed in TAL. Interestingly, isolated PIP4K?-positive cells were also localized to the collecting duct but were spatially differentiated from AQP2, which selectively stained principal cells (24). This suggested that PIP4K? was also localized to intercalated cells in cortical and medullary collecting ducts (Fig. 5, J?L).

PIP4K? has a distinct subcellular compartmentalization.

Tubule sections of medullary TAL showed a distinct concentration of PIP4K? around the lumen (Fig. 6, A?D). Analysis of a pool of cross-sectioned tubules with a diameter of 23?25 ?m gave an average fluorescence profile that indicated a sixfold increase of signal within 3 ?m of the apical membrane of tubule cells, compared with the basolateral membrane (Fig. 6E). Higher magnification of these cells suggested that PIP4K? might be present in a vesicular compartment (Fig. 6, C and D). To further investigate this compartmentalization, PIP4K? expression was studied in kidney-derived cell lines, but because endogenous levels of enzyme were not visible by immunocytochemistry in these cells, overexpressed protein levels were required for colocalization experiments. Cells expressing green fluorescent protein-tagged PIP4K? were stained with antibodies against different cellular markers (Fig. 7). PIP4K? was not seen to associate with markers for defined vesicular compartments such as peroxisomes (catalase), endosomes (EEA1, mannose 6-phosphate receptor), or lysosomes (lgp110) or with markers for structural components such as tubulin or actin (data not shown). In our experiments, PIP4K? was also not seen to associate with endoplasmic reticulum markers [calreticulin (Fig. 7A) and BiP] in contrast with the suggested endoplasmic reticulum localization seen by Itoh et al. (19). PIP4K? did, however, show a partial colocalization with a number of Golgi apparatus markers (golgin160, TGN38, p115, and p58K), most notably the cis-Golgi marker GM130 (Fig. 7B). Golgi dispersal by treatment of cells with Brefeldin A still retained partial colocalization of GM130 with the PIP4K? signal (Fig. 7C) but not with mannosidase II, a Golgi lumen protein, suggesting that the association was with the matrix component of this organelle (data not shown).

Lipid kinase activity of PIP4K?.

PIP4K, overexpressed in E. coli cells and purified by metal-affinity resin chromatography, was used as a source of enzyme for in vitro kinase assays. PIP4K activity was only observed using the PIP4K? isoform as the recombinant PIP4K? was inactive (Fig. 8A). Using immunoprecipitation experiments with recombinant protein, we were able to show that PIP4K? can associate with PIP4K? strongly enough to be selectively purified with a PIP4K?-specific antibody in vitro (Fig. 8B).


In this study, we investigated the expression, localization and associated biological activity of PIP4K?. We discovered a unique and restricted localization for this PIP4K in kidney tissue and suggest that this has implications for the physiological function of this isoform.

We have shown that the comparative mRNA expression levels of all three isoforms are significant in brain, where they have a different spatial distribution (1). PIP4K? is the most active of the three isoforms in vitro but has a comparably low mRNA expression in most of the tissues that we tested and is the most abundant isoform in the spleen, probably reflecting its role in hematocytes (17, 26). The PIP4K? isoform mRNA is highly expressed in heart and skeletal muscle cells, consistent with initial observations (6) and providing a link to insulin resistance (22). We have confirmed the original observation that PIP4K? is highly expressed in kidney (19) and also observe high mRNA expression levels in brain, heart, and testis compared with other tissues. Our detection of endogenous PIP4K? protein in tissues is consistent with these transcription levels, also suggesting that PIP4K? is abundant in the ovary and may be processed in heart tissue. The distribution of the PIP4Ks across a range of different tissues, and the observed differences in subcellular localization and intrinsic activity (36, 45), would suggest that specialized functions could be attributed to each and hence to the role of PtdIns(5)P in these locations.

Peptide analysis of the sequence of PIP4K? predicts that this protein would not be targeted to the endoplasmic reticulum or plasma membrane due to the absence of a recognized signal peptide, which is consistent with our observation that PIP4K?, when overexpressed in cells, is partially colocalized to the structural component of the Golgi apparatus. Roles for PtdIns3P and PtdIns4P in membrane trafficking are established (10, 32), but the recent study (25) of a Golgi-localized phospholipid-inositol phosphatase with a substrate preference for PtdIns5P presents the intriguing possibility that this phosphoinositide is also present in cellular vesicles. PIP4K? also has a role in actin remodeling during endocytic transport (30), and PtdIns5P levels have been associated with this and with vesicle translocation to the plasma membrane (38). PIP4K? could be recruited to the external surface of a specific microsomal compartment to modify the PtdIns5P signal or to synthesize PtdIns(4,5)P2.

The restricted expression of PIP4K? within the kidney may be significant within the context of the specialized function of different regions of the nephron. Our experiments indicate that PIP4K? is present in cells constituting the TAL and is also restricted to intercalated cells in the collecting duct and appears to have a similar subcellular localization in these cell types as that recently observed for members of the Arf GTPase family (11), which have known roles in membrane trafficking (34). These regions are predominantly concerned with homeostasis by active ion transport and pH regulation and contain a large number of channels and transporters specific to these tasks (for reviews see Refs. 20, 27). Phosphoinositide regulation of both the trafficking to the plasma membrane and the activity of various collecting duct-localized channels has been reported (14, 44), but a specific role for PIP4K? in this process has yet to be established.

PIP4K? has been shown to have PtdIns5P 4-kinase activity when immunoprecipitated from mammalian cells (19), and the recombinant protein, purified from E. coli, is inactive, suggesting that a eukaryotic modification to PIP4K? is required for kinase activation. However, due to the ability of the PIP4Ks to dimerize (4, 16, 35) in vivo, we also cannot rule out the possibility that the observed activity associated to PIP4K? is attributable to PIP4K heterodimers. This raises the possibility that PIP4K? is able to recruit PtdIns5P 4-kinase activity (in the form of PIP4K?) to specific compartments, based on the potential ability to act as a scaffolding protein.

The roles of PtdIns5P, PtdIns(4,5)P2, and hence the PIP4Ks, in kidney function are unknown. We have shown that PIP4K? is the predominant PIP4K in the kidney, and we suggest that its role may be related to its specific localization in this organ.


This study was supported by a Programme Grant (WT063581) from the Wellcome Trust and the Biotechnology and Biological Sciences Research Council.

We thank Dr. Patrick Lynch for practical advice, S?ren Nielsen for helpful suggestions, and Dr. Mihriban Tuna for technical expertise and analysis of biacore experiments.

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Keywords: phosphoinositide, phosphatidylinositol 5-phosphate 4-kinase, collecting duct, Golgi apparatus.

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