| Localization of the NO/cGMP-pathway in the cochlea of guinea pigs. | |
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MedLine Citation:
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PMID: 10416859 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The presence of nitric oxide synthase (NOS) in substructures of the cochlea of guinea pigs is an issue of current focus. Moreover, information concerning the localization of cells effected by the NO/cGMP-pathway are rare. Paraffin sections of guinea pig cochlea were incubated with specific antibodies to the three known NOS isoforms, soluble guanylyl cyclase (sGC) and cyclic guanosine-monophosphate (cGMP), the second messenger system of NO. While detection of inducible iNOS failed in all cochlear structures, expression of endothelial eNOS was found in the spiral ligament, in the stria vascularis, in cells of the organ of Corti, in nerve fibers and in some perikaryia of the spiral ganglion. The cochlear nerve showed an accentuated affinity for immunostaining in distal, basal segments of the cochlea. Neuronal bNOS was found predominantly in the endosteum of the modiolus and cochlea and was less intensively present in all perikaryia of the spiral ganglion and in the spiral ligament. Supporting cells of the organ of Corti and cells in the limbus spiralis displayed only modest immunostaining, while bNOS was not found in outer and inner hair cells. NOS detection was accompanied by immunoreactivity to sGC and to cGMP. The presence of NOS and its second messenger system gives evidence for a possible involvement in neurotransmission, regulation of the cochlear amplifier and in homeostasis. |
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Authors:
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O Michel; A Hess; W Bloch; E Stennert; J Su; K Addicks |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Hearing research Volume: 133 ISSN: 0378-5955 ISO Abbreviation: Hear. Res. Publication Date: 1999 Jul |
Date Detail:
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Created Date: 1999-09-23 Completed Date: 1999-09-23 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7900445 Medline TA: Hear Res Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 1-9 Citation Subset: IM |
Affiliation:
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Department of Oto-Rhino-Laryngology, University of Cologne, Germany. michel@uni-koeln.de |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cochlea / anatomy & histology, innervation, metabolism* Cyclic GMP / metabolism* Female Guanylate Cyclase / metabolism Guinea Pigs Immunohistochemistry Nitric Oxide / metabolism* Nitric Oxide Synthase / metabolism Nitric Oxide Synthase Type I Nitric Oxide Synthase Type II Nitric Oxide Synthase Type III Second Messenger Systems Solubility |
| Chemical | |
Reg. No./Substance:
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10102-43-9/Nitric Oxide; 7665-99-8/Cyclic GMP; EC 1.14.13.39/Nitric Oxide Synthase; EC 1.14.13.39/Nitric Oxide Synthase Type I; EC 1.14.13.39/Nitric Oxide Synthase Type II; EC 1.14.13.39/Nitric Oxide Synthase Type III; EC 4.6.1.2/Guanylate Cyclase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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