| Localization of organic anion transporting polypeptides in the rat and human ciliary body epithelium. | |
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MedLine Citation:
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PMID: 15652527 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: To identify and localize the expression of multispecific organic anion transporting polypeptides (Oatps/OATPs) in the ciliary body epithelium and to investigate their possible involvement in the transport of the antiglaucoma agent unoprostone. METHODS: Oatps/OATPs were detected by immunoblot analysis and by immunofluorescence microscopy in homogenized and fixed rat and human ciliary body samples using specific polyclonal antibodies. Transport of 3H-labelled unoprostone was measured in Oatp/OATP expressing Xenopus laevis oocytes. RESULTS: Immunoblots of ciliary body extracts were positive for rat Oatp1a4, Oatp1a5 and Oatp1b2 and for human OATP1A2, OATP1C1, OATP2B1, OATP3A1 and OATP4A1. Confocal immunofluorescence microscopy localized Oatp1a4 and Oatp1b2 as well as all immunoblot positive human OATPs at the basolateral plasma membrane of the non-pigmented rat and human ciliary body epithelium, respectively. However, for human OATPs additional regional differences in expression were found with OATP1A2 and OATP1C1 being expressed only in the pars plana of human ciliary body epithelium. Furthermore, OATP1C1, OATP3A1 and OATP4A1 were also expressed at the basolateral plasma membrane of the pars plana pigmented epithelium. And finally, deesterified unoprostone carboxylate was found to be transported by OATP1A2, OATP2B1 and OATP4A1 with approximate K(m)-values of 93, 91 and 132 microm, respectively. CONCLUSIONS: Several multispecific organic anion transporting polypeptides are expressed at the basolateral plasma membrane of the non-pigmented, and to a lesser extent also of the pigmented, epithelium in rat and human ciliary body. These Oatps/OATPs can account for the previously suggested 'liver-like' transport functions of mammalian ciliary body epithelium. |
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Authors:
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Bo Gao; Robert D Huber; Andreas Wenzel; Stephan R Vavricka; Manfred G Ismair; Charlotte Remé; Peter J Meier |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Experimental eye research Volume: 80 ISSN: 0014-4835 ISO Abbreviation: Exp. Eye Res. Publication Date: 2005 Jan |
Date Detail:
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Created Date: 2005-01-17 Completed Date: 2005-03-28 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370707 Medline TA: Exp Eye Res Country: England |
Other Details:
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Languages: eng Pagination: 61-72 Citation Subset: IM |
Affiliation:
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Division of Clinical Pharmacology and Toxicology, Department of Medicine, University Hospital Zurich, CH-8091 Zurich, Switzerland. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Aged, 80 and over Animals Antibody Specificity / immunology Antihypertensive Agents / analysis Biological Transport Cell Line Ciliary Body / immunology, metabolism* Dinoprost / analogs & derivatives*, analysis Epithelium / immunology, metabolism Female Fluorescent Antibody Technique / methods Humans Male Middle Aged Organic Anion Transport Polypeptide C / analysis, metabolism Organic Anion Transport Protein 1 / analysis, metabolism Organic Anion Transporters / analysis*, metabolism Peptides / metabolism Rats Rats, Inbred BN |
| Chemical | |
Reg. No./Substance:
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0/Antihypertensive Agents; 0/Organic Anion Transport Polypeptide C; 0/Organic Anion Transport Protein 1; 0/Organic Anion Transporters; 0/Peptides; 0/SLCO3A1 protein, human; 0/SLCO4A1 protein, human; 551-11-1/Dinoprost; 69553-75-9/isopropyl unoprostone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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