Document Detail

Localization of nucleic acid sequences by EM in situ hybridization using colloidal gold labels.
MedLine Citation:
PMID:  2476019     Owner:  NLM     Status:  MEDLINE    
It is possible to combine hybridization to specimens on electron-microscope grids of nucleic-acid probes labelled nonisotopically with immunogold detection of hybrid sites to map the position of target sequences rapidly and precisely. The basic technique is described, and examples are provided to illustrate the types of questions which can be approached in the general area of higher-order chromosome organization and function. A combination of two differentially labelled probes and two different-sized gold particles permits the simultaneous detection of closely linked or interspersed sequences.
B A Hamkalo; S Narayanswami; K Lundgren
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of anatomy     Volume:  185     ISSN:  0002-9106     ISO Abbreviation:  Am. J. Anat.     Publication Date:    1989 Jun-Jul
Date Detail:
Created Date:  1989-09-26     Completed Date:  1989-09-26     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0376312     Medline TA:  Am J Anat     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  197-204     Citation Subset:  IM    
Department of Molecular Biology and Biochemistry, University of California, Irvine 92717.
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MeSH Terms
Base Sequence*
Chromosome Mapping / methods*
Gold / diagnostic use*
Mice / genetics
Microscopy, Electron
Nucleic Acid Hybridization*
Xenopus laevis / genetics
Grant Support
Reg. No./Substance:
63231-63-0/RNA; 7440-57-5/Gold; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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