| Localization of multidomain adaptor proteins, p140Cap and vinexin, in the pancreatic islet of a spontaneous diabetes mellitus model, Otsuka Long-Evans Tokushima Fatty rats. | |
| | |
MedLine Citation:
|
PMID: 23325552 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
We have shown that two multidomain adaptor proteins, p140Cap and vinexin, interact with each other and are likely to be involved in neurotransmitter release. Because the basic molecular mechanism governing neurotransmitter and insulin secretion is conserved, these two proteins may also to play pivotal roles in insulin secretion. We therefore performed some characterization of p140Cap and vinexin in pancreas of a wild-type rat or a spontaneous type 2 diabetes mellitus (DM) model, the Otsuka Long-Evans Tokushima Fatty (OLETF) rat. These two proteins were detected in Wistar rat pancreas by Western blotting. Immunohistochemistry revealed that p140Cap and vinexin are enriched in β and α cells, respectively, in the rat pancreas. We then found that pancreatic islet structure was disorganized in the OLETF rat with hyperinsulinemia or with hyperglycemia, based on immunohistochemical analyses of vinexin. In β cells of these model rats, p140Cap was distributed in a cytoplasmic granular pattern as in the control rats, although its expression was reduced to various extents from cell to cell. These results may suggest possible involvement of p140Cap in insulin secretion, and reduction of p140Cap might be related to abnormal insulin secretion in DM. |
| | |
Authors:
|
Masahiro Yamauchi; Kaori Sudo; Hidenori Ito; Ikuko Iwamoto; Rika Morishita; Toshihiro Murai; Kazuo Kajita; Tatsuo Ishizuka; Koh-Ichi Nagata |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2013-1-17 |
Journal Detail:
|
Title: Medical molecular morphology Volume: - ISSN: 1860-1499 ISO Abbreviation: Med Mol Morphol Publication Date: 2013 Jan |
Date Detail:
|
Created Date: 2013-1-17 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101239023 Medline TA: Med Mol Morphol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Department of General Internal Medicine, Gifu University School of Medicine, Gifu, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Comparative sequential morphological analyses during in vitro chondrogenesis and osteogenesis of mes...
Next Document: Attention-deficit/hyperactivity disorder and obesity in US males and females, age 8-15?years: Nation...