Document Detail


Localization of the membrane-anchored MMP-regulator RECK at the neuromuscular junctions.
MedLine Citation:
PMID:  17953659     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nerve apposition on nicotinic acetylcholine receptor clusters and invagination of the post-synaptic membrane (i.e. secondary fold formation) occur by embryonic day 18.5 at the neuromuscular junctions (NMJs) in mouse skeletal muscles. Finding the molecules expressed at the NMJ at this stage of development may help elucidating how the strong linkage between a nerve terminal and a muscle fiber is established. Immunohistochemical analyses indicated that the membrane-anchored matrix metalloproteinase regulator RECK was enriched at the NMJ in adult skeletal muscles. Confocal and electron microscopy revealed the localization of RECK immunoreactivity in secondary folds and subsynaptic intracellular compartments in muscles. Time course studies indicated that RECK immunoreactivity becomes associated with the NMJ in the diaphragm at around embryonic day 18.5 and thereafter. These findings, together with known properties of RECK, support the hypothesis that RECK participates in NMJ formation and/or maintenance, possibly by protecting extracellular components, such as synaptic basal laminae, from proteolytic degradation.
Authors:
Satoshi Kawashima; Yukio Imamura; Ediriweera P S Chandana; Toru Noda; Rei Takahashi; Eijiro Adachi; Chiaki Takahashi; Makoto Noda
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-10-22
Journal Detail:
Title:  Journal of neurochemistry     Volume:  104     ISSN:  1471-4159     ISO Abbreviation:  J. Neurochem.     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2008-01-04     Completed Date:  2008-02-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985190R     Medline TA:  J Neurochem     Country:  England    
Other Details:
Languages:  eng     Pagination:  376-85     Citation Subset:  IM    
Affiliation:
Department of Molecular Oncology, Kyoto University Graduate School of Medicine, Sakyo-ku, Kyoto, Japan. mnoda@virus.kyoto-u.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn
Embryo, Mammalian
Gene Expression Regulation, Developmental / physiology*
Membrane Glycoproteins / metabolism*
Mice
Mice, Inbred ICR
Microscopy, Immunoelectron / methods
Muscle Proteins / metabolism
Muscle, Skeletal / cytology,  metabolism
Neuromuscular Junction / metabolism*,  ultrastructure
Chemical
Reg. No./Substance:
0/Membrane Glycoproteins; 0/Muscle Proteins; 0/Reck protein, mouse

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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