Document Detail

Localization of the membrane-anchored MMP-regulator RECK at the neuromuscular junctions.
MedLine Citation:
PMID:  17953659     Owner:  NLM     Status:  MEDLINE    
Nerve apposition on nicotinic acetylcholine receptor clusters and invagination of the post-synaptic membrane (i.e. secondary fold formation) occur by embryonic day 18.5 at the neuromuscular junctions (NMJs) in mouse skeletal muscles. Finding the molecules expressed at the NMJ at this stage of development may help elucidating how the strong linkage between a nerve terminal and a muscle fiber is established. Immunohistochemical analyses indicated that the membrane-anchored matrix metalloproteinase regulator RECK was enriched at the NMJ in adult skeletal muscles. Confocal and electron microscopy revealed the localization of RECK immunoreactivity in secondary folds and subsynaptic intracellular compartments in muscles. Time course studies indicated that RECK immunoreactivity becomes associated with the NMJ in the diaphragm at around embryonic day 18.5 and thereafter. These findings, together with known properties of RECK, support the hypothesis that RECK participates in NMJ formation and/or maintenance, possibly by protecting extracellular components, such as synaptic basal laminae, from proteolytic degradation.
Satoshi Kawashima; Yukio Imamura; Ediriweera P S Chandana; Toru Noda; Rei Takahashi; Eijiro Adachi; Chiaki Takahashi; Makoto Noda
Related Documents :
15802799 - Involvement of m3 muscarinic receptors in ach-induced increase in membrane-associated r...
3884749 - Release of acetylcholine from embryonic neurons upon contact with muscle cell.
19685119 - Effects of amitriptyline, a tricyclic antidepressant, on smooth muscle reactivity in is...
1206569 - Further studies on the control of ach sensitivity by muscle activity in the rat.
4011409 - Acetylcholine-sensitivity in fast and slow twitch muscle of normal and dystrophic (c57 ...
8124519 - Non-quantal release of acetylcholine affects polyneuronal innervation on developing rat...
2469539 - Intramural distribution of immunoreactive vasoactive intestinal polypeptide (vip), subs...
19705479 - Glutamine preserves skeletal muscle force during an inflammatory insult.
12402729 - Effects of halothane and isoflurane on the contraction, relaxation and energetics of ra...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-10-22
Journal Detail:
Title:  Journal of neurochemistry     Volume:  104     ISSN:  1471-4159     ISO Abbreviation:  J. Neurochem.     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2008-01-04     Completed Date:  2008-02-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985190R     Medline TA:  J Neurochem     Country:  England    
Other Details:
Languages:  eng     Pagination:  376-85     Citation Subset:  IM    
Department of Molecular Oncology, Kyoto University Graduate School of Medicine, Sakyo-ku, Kyoto, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Animals, Newborn
Embryo, Mammalian
Gene Expression Regulation, Developmental / physiology*
Membrane Glycoproteins / metabolism*
Mice, Inbred ICR
Microscopy, Immunoelectron / methods
Muscle Proteins / metabolism
Muscle, Skeletal / cytology,  metabolism
Neuromuscular Junction / metabolism*,  ultrastructure
Reg. No./Substance:
0/Membrane Glycoproteins; 0/Muscle Proteins; 0/Reck protein, mouse

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Kir4.1 channels regulate swelling of astroglial processes in experimental spinal cord edema.
Next Document:  Iron trafficking inside the brain.