Document Detail

Localization of a low Mr GTP-binding protein, rap1 protein, in plasma membranes and secretory granule membranes of rat parotid gland.
MedLine Citation:
PMID:  8007763     Owner:  NLM     Status:  MEDLINE    
Subcellular fractions were prepared from rat parotid gland by sequential centrifugation, Percoll gradient centrifugation and divalent-cation precipitation, and the localization of a low Mr GTP-binding protein, rap1 protein (rap1p) was analyzed by immunoblotting using a specific antibody. rap1p was found to be located in apical and basolateral plasma membranes, and secretory granule membranes in rat parotid gland. On the other hand, the beta gamma subunits of heterotrimeric Gt protein was localized in plasma membranes but not in granule membranes.
Y Kameyama; K Nagata; M Mizuno-Kamiya; Y Yokota; A Fujita; Y Nozawa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Life sciences     Volume:  55     ISSN:  0024-3205     ISO Abbreviation:  Life Sci.     Publication Date:  1994  
Date Detail:
Created Date:  1994-07-19     Completed Date:  1994-07-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375521     Medline TA:  Life Sci     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  213-9     Citation Subset:  IM    
Department of Oral Biochemistry, Asahi University School of Dentistry, Gifu, Japan.
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MeSH Terms
Cell Fractionation
Cell Membrane / chemistry
Cytoplasmic Granules / chemistry*
GTP-Binding Proteins / analysis*,  metabolism
Guanosine 5'-O-(3-Thiotriphosphate) / metabolism
Molecular Weight
Parotid Gland / chemistry*,  ultrastructure
Rats, Wistar
Subcellular Fractions / metabolism
rap GTP-Binding Proteins
Reg. No./Substance:
37589-80-3/Guanosine 5'-O-(3-Thiotriphosphate); EC 3.6.1.-/GTP-Binding Proteins; EC GTP-Binding Proteins

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