Document Detail

Localization of immunoglobulin G gamma-chain mRNA-expressing cells in the oviduct of laying and diethylstilbestrol-treated immature hens.
MedLine Citation:
PMID:  11121299     Owner:  NLM     Status:  MEDLINE    
The purpose of this study was to determine the synthetic sites of IgG in the chicken oviduct by localizing IgG gamma-chain mRNA (IgGgamma mRNA)-expressing cells and the effects of estrogen on their population. Paraffin sections of oviducal tissues from laying hens (approximately 57 weeks old) and immature hens (approximately 16 weeks old) with or without diethylstilbestrol (DES) treatment were hybridized by digoxigenin-labeled IgG riboprobes or immunostained for IgG gamma-chain (IgG). Immunoreactive IgG was present in some of the mucosal epithelial cells and the plasma cell-like cells in the stromal connective tissue in all segments of the oviduct. In contrast, IgGgamma mRNA expression was observed only in the plasma cell-like cells in the stromal connective tissues, but not in the cells of mucosal epithelium. In laying hens, the lower end of the oviduct, namely the vagina and uterovaginal junction, contained more IgGgamma mRNA-expressing cells than the other segments. Treatment of immature hens with DES for 3 or 6 days increased the population of both IgGgamma mRNA-expressing cells and IgG-containing cells in the oviducal stroma. These results indicate that IgG is locally produced by plasma cell-like cells in the stroma, but not by the cells of the mucosal epithelium, and estrogen may stimulate the infiltration of IgG-producing plasma cell-like cells into the oviducal stroma.
W M Zheng; J Izaki; S Furusawa; Y Yoshimura
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  General and comparative endocrinology     Volume:  120     ISSN:  0016-6480     ISO Abbreviation:  Gen. Comp. Endocrinol.     Publication Date:  2000 Dec 
Date Detail:
Created Date:  2001-01-24     Completed Date:  2001-02-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370735     Medline TA:  Gen Comp Endocrinol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  345-52     Citation Subset:  IM    
Copyright Information:
Copyright 2000 Academic Press.
Graduate School for International Development and Cooperation, Hiroshima University, Higashi-Hiroshima, 739-8528, Japan.
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MeSH Terms
Base Sequence
Chickens / immunology*
DNA, Complementary / chemistry
Diethylstilbestrol / pharmacology*
Epithelial Cells / immunology
Immunoglobulin G / genetics*
Immunoglobulin gamma-Chains / genetics*
In Situ Hybridization
Molecular Sequence Data
Mucous Membrane / cytology,  immunology
Oviducts / drug effects,  immunology*
Plasma Cells / immunology
RNA, Messenger / analysis*
Stromal Cells / immunology
Reg. No./Substance:
0/DNA, Complementary; 0/Immunoglobulin G; 0/Immunoglobulin gamma-Chains; 0/RNA, Messenger; 56-53-1/Diethylstilbestrol

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