Document Detail


Localization of human placental glucose transporter 1 during pregnancy. An immunohistochemical study.
MedLine Citation:
PMID:  8839758     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To elucidate the potential roles of glucose transporter 1 (GLUT1) in human placenta during pregnancy, we examined the localization of GLUT1 in human placenta at various stages by immunohistochemistry with an anti-GLUT1 antibody by use of both light and electron microscopy. Specific staining for GLUT1 was localized on the apical brush border and along the basal plasma membrane of the syncytiotrophoblasts. The staining at the apical side was more intense than that at the basal side during the early stages of gestation. In later gestational stages, however, the staining pattern at the apical side became blurred and the staining intensity at the basal side increased. The cytotrophoblasts, seen embedded in the basal part of the syncytiotrophoblasts, seemed to show immunoreactivity for GLUT1 along the plasma membranes at the light-microscopic level. However, immuno-electron microscopic analysis with either pre- or post-embedding methods revealed that specific staining for GLUT1 was hardly observed on the cytotrophoblasts, but the cytotrophoblasts were often surrounded by immunoreactive processes of syncytiotrophoblasts. The blood capillaries and erythrocytes in the stroma of placental villi were always immunoreactive for GLUT1 throughout pregnancy. These findings suggest that GLUT1 may play a vital role in human pregnancy.
Authors:
C Tadokoro; Y Yoshimoto; M Sakata; M Fujimiya; H Kurachi; E Adachi; T Maeda; A Miyake
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Histology and histopathology     Volume:  11     ISSN:  0213-3911     ISO Abbreviation:  Histol. Histopathol.     Publication Date:  1996 Jul 
Date Detail:
Created Date:  1997-02-19     Completed Date:  1997-02-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8609357     Medline TA:  Histol Histopathol     Country:  SPAIN    
Other Details:
Languages:  eng     Pagination:  673-81     Citation Subset:  IM    
Affiliation:
Department of Obstetrics and Gynecology, Osaka University Medical School, Japan.
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MeSH Terms
Descriptor/Qualifier:
Capillaries / ultrastructure
Chorionic Villi / ultrastructure
Coloring Agents
Erythrocytes / ultrastructure
Female
Glucose Transporter Type 1
Humans
Immunohistochemistry
Microscopy, Electron
Monosaccharide Transport Proteins / metabolism*
Placenta / metabolism*,  ultrastructure
Pregnancy / metabolism*
Trophoblasts / ultrastructure
p-Dimethylaminoazobenzene
Chemical
Reg. No./Substance:
0/Coloring Agents; 0/Glucose Transporter Type 1; 0/Monosaccharide Transport Proteins; 0/SLC2A1 protein, human; 60-11-7/p-Dimethylaminoazobenzene

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