Document Detail


Localization and hormonal regulation of endometrial matrix metalloproteinase-26 in the rhesus macaque.
MedLine Citation:
PMID:  22434853     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: The current understanding of hormonal regulation of matrix metalloproteinase-26 (MMP-26) in the primate endometrium is incomplete. The goal of this work was to clarify estrogen and progesterone regulation of MMP-26 in the endometrium of ovariectomized, hormone-treated rhesus macaques.
METHODS: Ovariectomized rhesus macaques (n= 66) were treated with estradiol (E(2)), E(2) plus progesterone, E(2) followed by progesterone alone or no hormone. Endometrium was collected from the hormone-treated animals during the early, mid- and late proliferative and secretory phases of the artificial menstrual cycle. MMP-26 expression was quantified by real-time PCR, and MMP-26 transcript and protein were localized by in situ hybridization and immunohistochemistry and correlated with estrogen receptor 1 and progesterone receptor (PGR).
RESULTS: MMP-26 was localized to glandular epithelium and was undetectable in the endometrial stroma and vasculature. MMP-26 transcript levels were minimal in the hormone-deprived macaques and treatment with E(2) alone did not affect MMP-26 levels. Treatment with progesterone both in the presence and absence of E(2) stimulated MMP-26 expression in the early and mid-secretory phases (P < 0.001). MMP-26 expression preceded decidualization of endometrial stroma. MMP-26 levels then declined to baseline in the late secretory phase (P < 0.01) despite continued E(2) plus progesterone treatment. Loss of detectable MMP-26 expression in the late secretory phase was correlated with late secretory phase loss of glandular epithelial PGR.
CONCLUSIONS: Endometrial MMP-26 expression is dependent on the presence of progesterone in the early secretory phase and then gradually becomes refractory to progesterone stimulation in the late secretory phase. In the macaque, MMP-26 is a marker of the pre-decidual, secretory endometrium. During the second half of the late secretory phase, and during decidualization, MMP-26 loses its response to progesterone concurrent with the loss of epithelial PGR. The decline in MMP-26 levels between the mid- and late secretory phases may play a role in the receptive window for embryo implantation.
Authors:
C C D Almeida-Francia; C S Keator; K Mah; L Holden; C Hergert; O D Slayden
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-03-20
Journal Detail:
Title:  Human reproduction (Oxford, England)     Volume:  27     ISSN:  1460-2350     ISO Abbreviation:  Hum. Reprod.     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-05-22     Completed Date:  2012-09-17     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  8701199     Medline TA:  Hum Reprod     Country:  England    
Other Details:
Languages:  eng     Pagination:  1723-34     Citation Subset:  IM    
Affiliation:
Department of Anatomy, Institute of Biosciences, Univ Estadual Paulista, Botucatu, São Paulo, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Animals
Endometrium / enzymology*
Epithelium / enzymology
Estradiol / pharmacology*
Female
Gene Expression Regulation, Enzymologic / drug effects*
Immunohistochemistry
Macaca mulatta*
Matrix Metalloproteinases, Secreted / analysis*,  genetics*
Menstrual Cycle / physiology
Ovariectomy
Progesterone / pharmacology*
RNA, Messenger / analysis
Real-Time Polymerase Chain Reaction
Receptors, Progesterone / analysis
Grant Support
ID/Acronym/Agency:
HD18185/HD/NICHD NIH HHS; RR000163/RR/NCRR NIH HHS; TW/HD-00668/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/RNA, Messenger; 0/Receptors, Progesterone; 50-28-2/Estradiol; 57-83-0/Progesterone; EC 3.4.24.-/Matrix Metalloproteinases, Secreted
Comments/Corrections

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