Document Detail


Localization of histidyl-tRNA synthetase (Jo-1) in human laryngeal epithelial carcinoma cell line (HEp-2 cells).
MedLine Citation:
PMID:  8929351     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The present study was designed to determine the subcellular localization of histidyl-tRNA synthetase (Jo-1) in human laryngeal epithelial carcinoma cell line (HEp-2 cells). Indirect immunofluorescence using commercial HEp-2 cells with human serum and human-affinity-purified anti-Jo-1 antibodies was performed using confocal microscopy. Anti-histidyl-tRNA-synthetase-positive sera showed distinct nuclear and cytoplasmic granular staining in HEp-2 cells. Affinity purified anti-Jo-1 produced an identical pattern to the whole serum, whereas the serum fraction that did not bind to the affinity column was negative by immunofluorescence on HEp-2 cells. Two commercial human anti-Jo-1-positive control sera and seven anti-Jo-1-positive sera from patients with myositis reproduced the nuclear and cytoplasmic granular pattern. We conclude that Jo-1 is present in cytoplasm and in intact nuclei from HEp-2 cells. The presence of tRNA synthetases in intact nuclei suggests that they have an unsuspected function in the nucleus.
Authors:
D Vázquez-Abad; J H Carson; N Rothfield
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cell and tissue research     Volume:  286     ISSN:  0302-766X     ISO Abbreviation:  Cell Tissue Res.     Publication Date:  1996 Dec 
Date Detail:
Created Date:  1997-02-20     Completed Date:  1997-02-20     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0417625     Medline TA:  Cell Tissue Res     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  487-91     Citation Subset:  IM    
Affiliation:
Division of Rheumatic Diseases, Department of Medicine, University of Connecticut School of Medicine, Farmington, CT 06030, USA. vazquez@panda.uchc.edu
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MeSH Terms
Descriptor/Qualifier:
Cell Nucleus / metabolism
Cytoplasm / metabolism
Hela Cells
Histidine-tRNA Ligase / metabolism*
Humans
Laryngeal Neoplasms / pathology
Myositis / blood,  immunology
Tumor Cells, Cultured
Grant Support
ID/Acronym/Agency:
AR20621/AR/NIAMS NIH HHS; AR37986/AR/NIAMS NIH HHS; K08-AR01954-01/AR/NIAMS NIH HHS
Chemical
Reg. No./Substance:
EC 6.1.1.21/Histidine-tRNA Ligase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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