Document Detail


Localization of the genes for major ribosomal RNA on chromosomes of the house musk shrew, Suncus murinus, at meiotic and mitotic cells by fluorescence in situ hybridization and silver staining.
MedLine Citation:
PMID:  9418261     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The genes for major ribosomal RNA were localized on chromosomes 5pter-p15, 9q64-qter, and 13q38-qter of the house musk shrew, Suncus murinus (Insectivora, Soricidae) by silver staining of mitotic metaphase and meiotic pachytene spreads and fluorescence in situ hybridization using the human 28S-RNA genes as a probe to mitotic metaphase spreads. The data presented indicate a correlation between sites of in situ hybridization and silver staining. The finding of nuclear materials in mitosis was in a good agreement with observation in meiosis: same chromosomes carried active NORs in both meiotic and mitotic cells.
Authors:
M B Rogatcheva; N A Serdyukova; L S Biltueva; P L Perelman; P M Borodin; S Oda; A S Graphodatsky
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Genes & genetic systems     Volume:  72     ISSN:  1341-7568     ISO Abbreviation:  Genes Genet. Syst.     Publication Date:  1997 Aug 
Date Detail:
Created Date:  1998-02-05     Completed Date:  1998-02-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9607822     Medline TA:  Genes Genet Syst     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  215-8     Citation Subset:  IM    
Affiliation:
School of Agricultural Sciences, Nagoya University, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Chromosome Mapping
Heterozygote
In Situ Hybridization, Fluorescence / methods*
Male
Meiosis*
Mitosis*
Nucleolus Organizer Region / genetics
Prophase / genetics
RNA, Ribosomal / genetics*
RNA, Ribosomal, 28S / genetics
Shrews / genetics*
Silver Staining / methods
Spermatocytes
Telomere
Chemical
Reg. No./Substance:
0/RNA, Ribosomal; 0/RNA, Ribosomal, 28S

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