Document Detail

Localization of gene and protein expressions of tumor necrosis factor-{alpha} (TNF), and TNF receptor types I and II in the bovine corpus luteum during the estrous cycle.
MedLine Citation:
PMID:  21551345     Owner:  NLM     Status:  Publisher    
One of the many roles of tumor necrosis factor-α (TNF) is to control mammalian corpus luteum (CL) prostaglandin synthesis and apoptotic cell death. Here, the cellular localization of TNF and its type I (TNF-RI) and type II (TNF-RII) receptors in bovine luteal tissue were analyzed using in situ hybridization, immunohistochemistry, and quantitative real-time PCR. Transcripts for TNF were expressed in bovine CL throughout the estrous cycle, but were significantly more abundant (P < 0.01) at the regressed luteal stage than at the other stages. Localization of TNF transcripts and protein were observed in large and small bovine luteal cells, as well as in immune cells. Moreover, transcripts for TNF-RI and TNF-RII were expressed in bovine CL throughout the estrous cycle. The abundance of TNF-RII transcripts was greater (P < 0.01) at the regressed luteal stage than at the other stages, whereas TNF-RI transcript abundance did not significantly change. Expression of TNF-RI and TNF-RII transcripts and proteins were observed in both the large and small luteal cells, and the proteins were also expressed in the immune cells and vascular endothelial cells. These results suggest that TNF sources include immune cells, as well as large and small luteal cells, and that TNF-RI and TNF-RII are present in the luteal cells of the bovine CL.
Ryosuke Sakumoto; Margarete Vermehren; Rebecca A Kenngott; Kiyoshi Okuda; Fred Sinowatz
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-5-6
Journal Detail:
Title:  Journal of animal science     Volume:  -     ISSN:  1525-3163     ISO Abbreviation:  -     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-5-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8003002     Medline TA:  J Anim Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Reproductive Biology Research Unit, National Institute of Agrobiological Sciences, Ibaraki 305-0901, Japan.
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