Document Detail

Localization of the gene coding for ventricular myosin regulatory light chain (MYL2) to human chromosome 12q23-q24.3.
MedLine Citation:
PMID:  1386340     Owner:  NLM     Status:  MEDLINE    
Human myosin light chain-2 (MYL2) is an important protein involved in the regulation of myosin ATPase activity in smooth muscle. In cardiac muscle, the precise role of MYL2 is not well understood; however, an increase in ventricular MYL2 is observed during myocardial hypertrophy in cardiac patients with valve stenosis. The chromosomal location of the gene coding for MYL2 was identified using a cloned cDNA for human MYL2. Southern blot analysis of DNA from a human/rodent somatic cell hybrid mapping panel showed that the BamHI fragment that hybridized with this cDNA probe was concordant with chromosome 12. The 768-bp cDNA was hybridized to human metaphase chromosomes. The results revealed a significant clustering of silver grains over chromosome 12 bands q23-q24.3, indicating that the gene coding for MYL2 is located in this region.
M J Macera; P Szabo; R Wadgaonkar; M A Siddiqui; R S Verma
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Genomics     Volume:  13     ISSN:  0888-7543     ISO Abbreviation:  Genomics     Publication Date:  1992 Jul 
Date Detail:
Created Date:  1992-08-28     Completed Date:  1992-08-28     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8800135     Medline TA:  Genomics     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  829-31     Citation Subset:  IM    
Division of Genetics, Long Island College Hospital, Brooklyn, New York 11201.
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MeSH Terms
Cardiomegaly / genetics,  metabolism
Chromosome Mapping
Chromosomes, Human, Pair 12*
DNA / genetics
Heart Ventricles / metabolism
Hybrid Cells
Myosins / genetics*,  metabolism
Grant Support
Reg. No./Substance:
9007-49-2/DNA; EC

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