Document Detail


Localization of gamma-rays induced chromatid breaks using a three consecutive staining technique.
MedLine Citation:
PMID:  340939     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Three staining techniques (Giemsa, Q-banding and R-banding) are used consecutively to localize the breakage points in chromosomes of human lymphocytes, irradiated during G2-phase with gamma-rays, at doses ranging from 50 to 200 rad. The large majority, about 85% of the breaks, occurs at the interbands, between R- and Q-bands. The discrepancy of this result, with regard to previously reported ones, is attributed to the strong bias of analysis when only one staining technique is used.
Authors:
C Dubos; E V Pequignot; B Dutrillaux
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Mutation research     Volume:  49     ISSN:  0027-5107     ISO Abbreviation:  Mutat. Res.     Publication Date:  1978 Jan 
Date Detail:
Created Date:  1978-03-29     Completed Date:  1978-03-29     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  0400763     Medline TA:  Mutat Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  127-31     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Azure Stains
Chromatids / radiation effects,  ultrastructure*
Chromosome Aberrations*
Cytological Techniques
Gamma Rays
Quinacrine
Chemical
Reg. No./Substance:
0/Azure Stains; 83-89-6/Quinacrine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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