| Localization and function of mSpindly during mouse oocyte meiotic maturation. | |
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MedLine Citation:
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PMID: 20505367 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Spindly was first identified in Drosophila; its homologues are termed SPDL-1 in Caenorhabditis elegans and Hs Spindly/hSpindly in humans. In all species, Spindly and its homologues function by recruiting dynein to kinetochores and silencing SAC in mitosis of somatic cells. Depletion of Spindly causes an extensive metaphase arrest during somatic mitoses in Drosophila, C. elegans and humans. In Drosophila, Spindly is required for shedding of Rod and Mad2 from the kinetochores in metaphase; in C. elegans, SPDL-1 presides over the recruitment of dynein and MDF-1 to the kinetochores; in humans, Hs Spindly is required for recruiting both dynein and dynactin to kinetochores but it is dispensable for removal of checkpoint proteins from kinetochores. The present study was designed to investigate the localization and function of the Spindly homologue (mSpindly) during mouse oocyte meiotic maturation by immunofluorescent analysis, and by overexpression and knockdown of mSpindly. We found that mSpindly was typically localized to kinetochores when chromatin condensed into chromosomes after GVBD. In metaphase of both first meiosis and second meiosis, mSpindly was localized not only to kinetochores but also to the spindle poles. Overexpression of mSpindly did not affect meiotic progression, but its depletion resulted in an arrest of the pro-MI/MI stage, failure of anaphase entry and subsequent polar body emission, and in abnormal spindle morphology and misaligned chromosomes. Our data suggest that mSpindly participates in SAC silencing and in spindle formation as a recruiter and/or a transporter of kinetochore proteins in mouse oocytes, but that it needs to cooperate with other factors to fulfill its function. |
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Authors:
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Qing-Hua Zhang; Liang Wei; Jing-Shan Tong; Shu-Tao Qi; Sen Li; Xiang-Hong Ou; Ying-Chun Ouyang; Yi Hou; Li-Gou An; Heide Schatten; Heide Schatten; Qing-Yuan Sun |
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Publication Detail:
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Type: Journal Article Date: 2010-06-01 |
Journal Detail:
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Title: Cell cycle (Georgetown, Tex.) Volume: 9 ISSN: 1551-4005 ISO Abbreviation: Cell Cycle Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2011-05-17 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101137841 Medline TA: Cell Cycle Country: United States |
Other Details:
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Languages: eng Pagination: 2230-6 Citation Subset: IM |
Affiliation:
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College of Life Sciences; Shandong Normal University; Jinan, Shandong; China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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