Document Detail

Localization of fibronectin in megakaryocytes of fetal liver.
MedLine Citation:
PMID:  2916757     Owner:  NLM     Status:  MEDLINE    
Antibodies specific for fibronectin were utilized to determine the sites of localization in the liver during development. The livers of fetal rats from each of gestation days 11-19, and from days 1 and 8 postpartum, were studied by fluorescence microscopy. Fibronectin was localized predominantly in megakaryocytes and megakaryocyte precursors, and to a lesser extent in the extracellular matrix surrounding blood vessels and between hepatocytes and sinusoids. The cytoplasm of megakaryocytes and their precursors displayed bright fluorescence but their nuclei were negative for fibronectin. Hepatocytes had negative or faint fluorescence. Megakaryocytes were present in the liver from day 12, and were numerous from day 13 through most of the rest of gestation. The relative numbers of megakaryocytes decreased in later gestation; at 8 days postpartum only a few were observed per section. Hepatic megakaryocytes appeared before megakaryocytes were established in spleen and bone marrow. The early and persistent high levels of fibronectin in hepatic megakaryocytes, in the absence of comparable localization within hepatocytes, leads us to the hypothesis that megakaryocytes are important in establishing circulating fibronectin levels in the fetus. Similarly, bone marrow megakaryocytes may contribute to circulating fibronectin in the adult.
D E Bockman; A K Gulati
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Anatomical record     Volume:  223     ISSN:  0003-276X     ISO Abbreviation:  Anat. Rec.     Publication Date:  1989 Jan 
Date Detail:
Created Date:  1989-03-23     Completed Date:  1989-03-23     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0370540     Medline TA:  Anat Rec     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  90-4     Citation Subset:  IM    
Department of Anatomy, Medical College of Georgia, Augusta 30912-2000.
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MeSH Terms
Bone Marrow Cells
Fetus / analysis*
Fibronectins / analysis*
Liver / analysis,  cytology,  embryology*
Megakaryocytes / analysis*,  physiology
Microscopy, Electron
Rats, Inbred Strains
Spleen / cytology
Reg. No./Substance:

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