Document Detail


Localization of factor VIIIC: antigen in guinea-pig tissues and isolated liver cell fractions.
MedLine Citation:
PMID:  6424698     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Factor VIIIC:antigen (VIII:CAg) was estimated in guinea-pig tissues by an immunoradiometric assay using a human inhibitor antibody. In homogenized guinea-pig tissues, VIII:CAg was shown to be stable and to be predominantly located in the liver (9 +/- 1.2 units; mean +/- SEM, n = 8). Lesser amounts were detected in spleen (1.3 +/- 0.02 units), lung (0.6 +/- 0.07) and kidney (0.4 +/- 0.06). In isolated liver cell fractions separated by centrifugal elutriation VIII:CAg was mainly detected in the hepatocyte fraction (0.3 +/- 0.07 units/10(8) cells;mean +/- SEM, n = 5) and in lesser amounts in the endothelial (0.02 +/- 0.01 units/10(8) cells) and the Kupffer cell fractions (0.05 +/- 0.02 units/10(8) cells). The liver concentration of VIII:CAg was (0.17 +/- 0.02 units/g) which was 20% of the plasma concentration (0.96 +/- 0.01 units/ml, n = 8) suggesting that VIII:CAg may not be stored in the liver but is rapidly exported following synthesis.
Authors:
D A Kelly; J A Summerfield; E G Tuddenham
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  British journal of haematology     Volume:  56     ISSN:  0007-1048     ISO Abbreviation:  Br. J. Haematol.     Publication Date:  1984 Apr 
Date Detail:
Created Date:  1984-06-07     Completed Date:  1984-06-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372544     Medline TA:  Br J Haematol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  535-43     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens / analysis*
Endothelium / immunology
Factor VIII / analysis,  immunology*
Guinea Pigs
Kidney / immunology
Kupffer Cells / immunology
Liver / cytology,  immunology*,  ultrastructure
Lung / immunology
Male
Radioimmunoassay
Spleen / immunology
Chemical
Reg. No./Substance:
0/Antigens; 9001-27-8/Factor VIII

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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