| Localization and expression of thyroid hormone receptors normal and neoplastic human thyroid. | |
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MedLine Citation:
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PMID: 14759075 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of this study was to investigate the regional expression of thyroid hormone nuclear receptor forms (TR(alpha) and TR(beta)) and isoform (TR(alpha1) and TR(beta2)) mRNAs in normal and neoplastic (benignant and malignant) human thyroid tissue. Tumor specimens from patients with thyroid carcinomas (papillary: 5 cases; follicular: 5 cases; anaplastic: 2 cases), thyroid follicular adenomas (7 cases) and tissue from normal thyroid glands (12 cases) were analyzed by in situ hybridization and semiquantitative RT-PCR for the expression of TR(alpha1) and beta, as well as for the isoform alpha2 that does not bind the hormone. In normal tissues, TR(alpha2) was expressed at lower levels compared to TR(alpha1) (alpha1/alpha2 = 4.3). In papillary and follicular carcinomas, the expression of TR(alpha1) and TR(beta) did not change as compared with normal thyroid tissue and adenomas (0.87 +/- 0.15 SD vs 0.89 +/- 0.17 densitometric units, DU, and 0.15 +/- 0.02 vs 0.14 +/- 0.03 DU, respectively). However, the expression of TR(alpha2) was significantly higher in differentiated carcinomas compared to normal thyroid tissue and adenomas (0.47 +/- 0.05 vs 0.20 +/- 0.05 DU, p < 0.05) with alpha1/alpha2 = 1.4. In anaplastic carcinoma all TRs were absent. We concluded that both normal and pathological thyroid tissues, with the exception of anaplastic carcinoma, express all TRs in thyreocites and that differentiated thyroid carcinomas are associated in enhancing the expression of TR(alpha2) mRNA. |
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Authors:
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A Crescenzi; M F Graziano; E Carosa; E Papini; N Rucci; F Nardi; P Trimboli; A Calvanese; E A Jannini; M D'Armiento |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of endocrinological investigation Volume: 26 ISSN: 0391-4097 ISO Abbreviation: J. Endocrinol. Invest. Publication Date: 2003 Oct |
Date Detail:
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Created Date: 2004-02-04 Completed Date: 2004-09-07 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7806594 Medline TA: J Endocrinol Invest Country: Italy |
Other Details:
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Languages: eng Pagination: 1008-12 Citation Subset: IM |
Affiliation:
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Institute Regina Apostolorum, Albano, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenocarcinoma, Follicular
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genetics,
pathology Adult Blotting, Northern Carcinoma / genetics, metabolism Carcinoma, Papillary / genetics, pathology Female Gene Expression Regulation, Neoplastic Humans In Situ Hybridization RNA, Neoplasm / chemistry, genetics Receptors, Thyroid Hormone / biosynthesis, genetics, metabolism* Reverse Transcriptase Polymerase Chain Reaction Thyroid Gland / metabolism Thyroid Hormone Receptors alpha / biosynthesis, genetics, metabolism* Thyroid Hormone Receptors beta Thyroid Neoplasms / genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/RNA, Neoplasm; 0/Receptors, Thyroid Hormone; 0/Thyroid Hormone Receptors alpha; 0/Thyroid Hormone Receptors beta |
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