| Localization and effects of calcitonin gene-related peptide in the testicular vasculature of the rat. | |
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MedLine Citation:
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PMID: 9283951 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Using laser Doppler flowmetry, the effects of unilateral intratesticular injection of calcitonin gene-related peptide (CGRP) and CGRP8-37, a CGRP-receptor antagonist, on right- and left-testicular blood flow and mean arterial pressure were studied on anesthetized adult rats. Calcitonin gene-related peptide in doses of 5 and 50 ng increased blood flow 37 +/- 11% (mean +/- SEM, P < 0.05) and 30 +/- 5% at 5 mm, but not 15 mm, away from the injection site, respectively. They did not influence mean arterial pressure nor blood flow in the contralateral testis. Five-hundred nanogram doses increased testicular blood flow in the injected testis at a point 15 mm away from the injection site (22 +/- 3%, P < 0.05) and caused a slight decrease in mean arterial pressure (-12 +/- 3%, P < 0.05). The highest dose, 5 micrograms, caused a large (-39 +/- 3%, P < 0.05) fall in mean arterial pressure within 1 minute after injection, and testicular blood flow was reduced in both the injected (-9 +/- 2%, P < 0.05, 15 mm away from injection site) and contralateral testis (-20 +/- 5%, P < 0.05). Pretreatment with 500 ng of the receptor antagonist, CGRP8-37, did not significantly attenuate the blood flow increasing affect of 50 ng CGRP, nor did 50 micrograms CGRP 8-37 (given alone) influence basal testicular blood flow in the injected testis. Using Immunohistochemistry, CGRP-containing nerves were observed in the superior and interior spermatic nerves, in the testicular artery, and in the veins leaving the testis but not in intratesticular blood vessels. Conclusions: 1) CGRP is a potent vasodilator in the testicular vasculature and it may be involved in the local regulation of testicular blood flow: 2) the testis has limited capacity to autoregulate and is consequently unable to maintain a constant testicular blood flow during large and rapid reductions in blood pressure, and 3) the local and systemic effects of vasodilators act in opposite directions in the testis. |
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Authors:
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E Lissbrant; O Collin; A Bergh |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of andrology Volume: 18 ISSN: 0196-3635 ISO Abbreviation: J. Androl. Publication Date: 1997 Jul-Aug |
Date Detail:
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Created Date: 1997-10-10 Completed Date: 1997-10-10 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8106453 Medline TA: J Androl Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 385-92 Citation Subset: IM |
Affiliation:
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Department of Pathology, Umeå University, Sweden. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Calcitonin Gene-Related Peptide / administration & dosage, metabolism*, pharmacology Immunohistochemistry Male Rats Rats, Sprague-Dawley Regional Blood Flow / drug effects Testis / blood supply*, drug effects, metabolism |
| Chemical | |
Reg. No./Substance:
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83652-28-2/Calcitonin Gene-Related Peptide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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