Document Detail


Localization and differential expression of FMRFamide-like immunoreactivity in the nematode Ascaris suum.
MedLine Citation:
PMID:  8349852     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
By immunocytochemical and immunohistochemical methods, FMRFamide-like immunoreactivity (FLI) was localized to many neurons and processes in the Ascaris nervous system, including the head, tail, and lateral lines. Some of these cells were identified; they included sensory neurons, interneurons, and motor neurons. FLI was also present in the pharyngeal neurons and in their varicosities near the surface of the pharynx. By HPLC analysis of extracts, only a subset of the FMRFamide-like peptides (FLPs) expressed in Ascaris heads, and heads from which the pharynx had been removed, were expressed in the pharynx. Furthermore, FLPs appeared to be differentially expressed in female heads and tails and male heads and tails. Acetone and acid methanol differentially extracted subforms of FLI from Ascaris heads and from C. elegans.
Authors:
C Cowden; P Sithigorngul; P Brackley; J Guastella; A O Stretton
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of comparative neurology     Volume:  333     ISSN:  0021-9967     ISO Abbreviation:  J. Comp. Neurol.     Publication Date:  1993 Jul 
Date Detail:
Created Date:  1993-09-10     Completed Date:  1993-09-10     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0406041     Medline TA:  J Comp Neurol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  455-68     Citation Subset:  IM    
Affiliation:
Department of Zoology, University of Wisconsin, Madison 53706.
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MeSH Terms
Descriptor/Qualifier:
Animals
Ascaris suum / metabolism*
Chromatography, High Pressure Liquid
FMRFamide
Female
Immunohistochemistry
Invertebrate Hormones / metabolism
Male
Motor Neurons / metabolism
Neurons / metabolism
Neuropeptides / metabolism*
Pharynx / innervation
Radioimmunoassay
Tissue Distribution
Grant Support
ID/Acronym/Agency:
AI 15429/AI/NIAID NIH HHS; AI 20355/AI/NIAID NIH HHS; NS07954/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Invertebrate Hormones; 0/Neuropeptides; 64190-70-1/FMRFamide

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