| LOCALIZATION OF CYTOPLASMIC NUCLEIC ACID DURING GROWTH AND ENCYSTMENT OF ENTAMOEBA INVADENS. | |
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MedLine Citation:
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PMID: 14128044 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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With a modified color-translating ultraviolet microscope, the distribution of material showing an absorption maximum at 265 mmicro was studied in samples from whole cultures of Entamoeba invadens at intervals during growth and from cysts allowed to mature under controlled conditions. Absorption by the cytoplasm in general gradually increased as trophozoites approached the period of maximum encystment. In late trophozoites and precystic forms, the absorbing material was concentrated into small bodies which coalesced to form large crystalloids of very high specific absorption. Maximum crystallization occurred in early cysts, where cytochemical tests have shown the large crystalloids to be ribonucleoprotein. Electron micrographs show that the crystalloids are composed of particles 200 to 300 A in diameter. During cyst maturation the amount of absorbing material per cyst is not visibly reduced, but the large bodies fragment into smaller units until finally there is only a very high diffuse absorption over the entire cyst. From these and other results the hypothesis is advanced that the large crystalloids ("chromatoid bodies") are a manifestation of a special parasite-host adaptive mechanism; ribonucleoprotein is synthesized under favorable conditions, crystallized in the resistant cyst stage, and dispersed in the newly excysted amebae thereby enabling them to establish themselves in a new host by a period of quick growth. |
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Authors:
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D C BARKER; G SVIHLA |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of cell biology Volume: 20 ISSN: 0021-9525 ISO Abbreviation: J. Cell Biol. Publication Date: 1964 Mar |
Date Detail:
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Created Date: 1964-07-01 Completed Date: 1996-12-01 Revised Date: 2010-09-20 |
Medline Journal Info:
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Nlm Unique ID: 0375356 Medline TA: J Cell Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 389-98 Citation Subset: OM |
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| MeSH Terms | |
Descriptor/Qualifier:
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Bacteriological Techniques* Cell Biology* Cell Division* Cytoplasm* Cytoplasmic Granules* Entamoeba* Histocytochemistry* Microscopy* Microscopy, Electron* Mitochondria* Nucleic Acids* Nucleoproteins* RNA* Ribosomes* Spectrum Analysis* Ultraviolet Rays* |
| Chemical | |
Reg. No./Substance:
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0/Nucleic Acids; 0/Nucleoproteins; 63231-63-0/RNA |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): J Cell Biol ISSN: 0021-9525 ISSN: 1540-8140 Publisher: The Rockefeller University Press |
Article Information Download PDF  Copyright © 1964 by The Rockefeller Institute Press Received Day: 22 Month: 5 Year: 1963 Print publication date: Day: 1 Month: 3 Year: 1964 Volume: 20 Issue: 3 First Page: 389 Last Page: 398 ID: 2106411 PubMed Id: 14128044 |
| LOCALIZATION OF CYTOPLASMIC NUCLEIC ACID DURING GROWTH AND ENCYSTMENT OF ENTAMOEBA INVADENS | |
| Douglas Cameron Barker | |
| George Svihla | |
| From the Division of Biological and Medical Research, Argonne National Laboratory, Argonne, Illinois. |
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| Dr. Barker's present address is the Department of Zoology, University of Edinburgh, Edinburgh, Scotland |
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