Document Detail


Localization of cytochrome oxidase and the 2-methyl branched-chain enoyl CoA reductase in muscle and hypodermis of Ascaris suum larvae and adults.
MedLine Citation:
PMID:  9267425     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Rabbit lung-derived third-stage larvae (L3) of Ascaris suum are aerobic and cyanide sensitive but also contain many enzymes specific to anaerobic pathways. To localize these enzymes, diaminobenzidine (DAB) staining for cytochrome oxidase (COX) and immunogold labeling for 2-methylbutyryl enoyl CoA reductase (ECR) were performed on sections of hypodermis and muscle of adults and larvae of A. suum and visualized by transmission electron microscopy. As predicted, adult hypodermal and muscle mitochondria did not exhibit COX staining; however, hypodermal and muscle mitochondria of the L3 and fourth-stage larvae (L4) were DAB positive. In contrast, hypodermal mitochondria from the adult, L3, and L4 did not exhibit ECR immunoreactivity, whereas mitochondria from muscle of all 3 were ECR positive. These observations suggest that both the ECR and COX are colocalized in muscle mitochondria of the L3.
Authors:
B Mei; R Komuniecki; P R Komuniecki
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of parasitology     Volume:  83     ISSN:  0022-3395     ISO Abbreviation:  J. Parasitol.     Publication Date:  1997 Aug 
Date Detail:
Created Date:  1997-09-02     Completed Date:  1997-09-02     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  7803124     Medline TA:  J Parasitol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  760-3     Citation Subset:  IM    
Affiliation:
Department of Biology, University of Toledo, Ohio 43606-3390, USA.
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MeSH Terms
Descriptor/Qualifier:
3,3'-Diaminobenzidine
Animals
Ascaris suum / enzymology*,  ultrastructure
Electron Transport Complex IV / analysis*
Fatty Acid Desaturases / analysis*
Female
Immunohistochemistry
Larva / enzymology
Microscopy, Electron
Mitochondria, Muscle / enzymology
Rabbits
Staining and Labeling
Grant Support
ID/Acronym/Agency:
AI18427/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
91-95-2/3,3'-Diaminobenzidine; EC 1.14.19.-/Fatty Acid Desaturases; EC 1.9.3.1/Electron Transport Complex IV

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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