| Localization of collagen XVIII and endostatin in the human eye. | |
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MedLine Citation:
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PMID: 15875362 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: To investigate the localization of endostatin, a potent angiogenesis inhibitor, and its progenitor collagen XVIII in the human eye. METHODS: Twelve normal human eyes were investigated. Immunohistochemistry of the anterior and posterior eye segment was performed using a polyclonal endostatin and collagen XVIII antibody and a monoclonal collagen XVIII antibody. Specificity of the antibodies was confirmed by Western blot analysis. RESULTS: The antibody against collagen XVIII stained Bowman's membrane, the lens capsule, the trabecular meshwork, and all epithelial and endothelial basal membranes in the anterior and posterior eye segment. In contrast, the antibody against endostatin showed a more distinct staining pattern. Intense intracellular staining for endostatin was present in the lens epithelium and in the non-pigmented epithelium of the ciliary body. Extracellular presence of endostatin could be detected in the lens capsule and all border membranes lining the aqueous humor including the anterior surface of the iris. The choroid was unstained. In the retina, staining was restricted to the inner limiting membrane and to endothelial cells of larger vessels. CONCLUSIONS: Our results show that there is a ring of specifically endostatin expressing structures forming a "barrier" around the anterior chamber and the vitreous. This might physiologically prevent vessels from sprouting into these avascular compartments. |
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Authors:
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Anne V Ohlmann; Andreas Ohlmann; Ulrich Welge-Lüssen; Christian A May |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Current eye research Volume: 30 ISSN: 0271-3683 ISO Abbreviation: Curr. Eye Res. Publication Date: 2005 Jan |
Date Detail:
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Created Date: 2005-05-06 Completed Date: 2005-05-24 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8104312 Medline TA: Curr Eye Res Country: England |
Other Details:
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Languages: eng Pagination: 27-34 Citation Subset: IM |
Affiliation:
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University Eye Hospital, Ludwig-Maximilians University, Munich, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Aged, 80 and over Blotting, Western Collagen Type XVIII / metabolism* Endostatins / metabolism* Eye / metabolism* Female Humans Immunohistochemistry Male Middle Aged |
| Chemical | |
Reg. No./Substance:
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0/Collagen Type XVIII; 0/Endostatins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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