Document Detail

Localization of c-kit and stem cell factor (SCF) in ovarian follicular epithelium of a lizard, Podarcis s. sicula.
MedLine Citation:
PMID:  20850173     Owner:  NLM     Status:  In-Data-Review    
We performed an immunohistochemical study to determine the immunolocalization of c-kit and stem cell factor (SCF) in ovarian follicles during the reproductive cycle of the lizard, Podarcis s. sicula. Follicles were serially cut and used for histological and histochemical characterization and also for immunohistochemical detection of both c-kit and SCF. C-kit and SCF were localized in the follicles with a differing pattern with regard to the stage of sexual cycle or the cell type forming the follicular epithelium (granulosa). In pre-reproductive follicles, where the granulosa consists of three main different cytotypes, the c-kit receptor was prevalently localized on the plasmalemma of small cells, although some pyriform and intermediate cells also appeared positive. C-kit was also localized in the theca. In pre-reproductive follicles, SCF was markedly observed in the cytoplasm of some pyriform cells. Small cells and theca also stained moderately positive, whereas the intermediate cells were mostly negative. In reproductive follicles, where granulosa cells are morphologically rearranged, c-kit was observed in small cells and in some thecal elements, while SCF was weakly immunostained. At the site of follicular layer invaginations evident c-kit/SCF immunostaining was observed in the granulosa epithelium and in the theca. These observations suggest that the expression of c-kit and SCF changes as a function of follicular development and may reflect the involvement of this system in the maturation of the oocyte.
Franca Raucci; Maria Maddalena Di Fiore
Related Documents :
1688793 - Follicle-stimulating hormone inhibits the constitutive release of insulin-like growth f...
11408113 - Receptors for insulin-like growth factor-i and tumor necrosis factor-alpha are hormonal...
24109643 - A quantitative analysis of f-actin features and distribution in fluorescence microscopy...
Publication Detail:
Type:  Journal Article     Date:  2010-09-17
Journal Detail:
Title:  Acta histochemica     Volume:  113     ISSN:  1618-0372     ISO Abbreviation:  Acta Histochem.     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-06-07     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370320     Medline TA:  Acta Histochem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  647-55     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier GmbH. All rights reserved.
Dipartimento di Scienze della Vita, Second University of Naples, Via Vivaldi 43, 81100 Caserta, Italy.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Inter-individual variability of effect of 7 low molecular weight antithrombin-dependent anticoagulan...
Next Document:  Correlates of the preoperative level of CA125 at presentation of ovarian cancer.