Document Detail


Localization of binding sites of Ulex europaeus I, Helix pomatia and Griffonia simplicifolia I-B4 lectins and analysis of their backbone structures by several glycosidases and poly-N-acetyllactosamine-specific lectins in human breast carcinomas.
MedLine Citation:
PMID:  8897074     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Several studies have shown the deletion of blood group A or B antigens and the accumulation of H antigens in human breast carcinomas. Other studies have independently demonstrated that the binding sites of lectins such as Helix pomatia agglutinin (HPA) and Griffonia simplicifolia agglutinin I-B4 (GSAI-B4) are highly expressed in these cells. In order to clarify the molecular mechanisms of malignant transformation and metastasis of carcinoma cells, it is important to understand the relationship between such phenotypically distinct events. For this purpose, we examined whether the binding sites of these lectins and Ulex europaeus agglutinin I (UEA-I) are expressed concomitantly in the same carcinoma cells and analyzed their backbone structures. The expression of the binding sites of these lectins was observed independently of the blood group (ABO) of the patients and was not affected by the histological type of the carcinomas. Observation of serial sections stained with these lectins revealed that the distribution of HPA binding sites was almost identical to that of GSAI-B4 in most cases. Furthermore, in some cases, UEA-I binding patterns were similar to those of HPA and GSAI-B4 but in other cases, mosaic staining patterns with these lectins were also observed, i.e., some cell clusters were stained with both HPA and GSAI-B4 but not with UEA-I and adjacent cell clusters were stained only with UEA-I. Digestion with endo-beta-galactosidase or N-glycosidase F markedly reduced the staining intensity of these lectins. Together with the reduction of staining by these lectins, reactivity with Griffonia simplicifolia agglutinin II appeared in carcinoma cells following endo-beta-galactosidase digestion. Among the lectins specific to poly-N-acetyllactosamine, Lycopersicon esculentum agglutinin (LEA) most vividly and consistently stained the cancer cells. Next to LEA, pokeweed mitogen agglutinin was also effective in staining these cells. Carcinoma cells reactive with these lectins corresponded well to those stained with both HPA and GSAI-B4, and in some cases, with UEA-I. These results demonstrate that the binding sites of UEA-I, HPA, and GSAI-B4 are expressed concomitantly in the same carcinoma cells and all carry linear and branched poly-N-acetyllactosamine on N-glycans, suggesting that the synthesis of this complex carbohydrate is one of the most important and basic processes leading to the malignant transformation of cells, invasion, and metastasis of carcinoma cells.
Authors:
N Ito; S Imai; S Haga; C Nagaike; Y Morimura; K Hatake
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Histochemistry and cell biology     Volume:  106     ISSN:  0948-6143     ISO Abbreviation:  Histochem. Cell Biol.     Publication Date:  1996 Sep 
Date Detail:
Created Date:  1997-02-11     Completed Date:  1997-02-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9506663     Medline TA:  Histochem Cell Biol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  331-9     Citation Subset:  IM    
Affiliation:
Department of Legal Medicine, Nara Medical University, Japan.
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MeSH Terms
Descriptor/Qualifier:
ABO Blood-Group System / analysis
Adenocarcinoma / pathology*
Adenocarcinoma, Scirrhous / pathology*
Antibodies, Monoclonal
Binding Sites
Breast / pathology
Breast Neoplasms / pathology*
Carbohydrate Sequence
Carcinoma, Papillary / pathology*
Female
Glycoside Hydrolases
Humans
Immunohistochemistry
Lectins* / chemistry*
Molecular Sequence Data
Plant Lectins*
Polysaccharides / analysis*
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/ABO Blood-Group System; 0/Antibodies, Monoclonal; 0/Helix lectin; 0/Lectins; 0/Plant Lectins; 0/Polysaccharides; 0/Ulex europaeus lectins; 82441-98-3/poly-N-acetyllactosamine; EC 3.2.1.-/Glycoside Hydrolases

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