Document Detail


Localization of anti-mitochondrial antibody in experimental canine myocardial infarcts.
MedLine Citation:
PMID:  6935688     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Alterations in cell and subcellular membrane integrity occur during evolving ischemic myocardial injury. We tested the hypothesis that an antibody against human liver mitochondria [anti-mitochondrial antibody developing in a patient with primary biliary cirrhosis] could identify altered cell membrane integrity in experimental canine myocardial infarcts. The proximal left anterior descending coronary arteries of 12 dogs were ligated and 1 hr later 131I-labeled F(ab')2 fragments from either a control human IgG (6 dogs) or anti-mitochondrial IgG (6 dogs) were injected. The 131I-labeled F(ab')2 anti-mitochondrial fragments concentrated maximally in the central infarct subendocardium [infarct-to-normal ratio of 9.2 +/- 3.5 (mean +/- SD) vs. 4.6 +/- 3.3 for control F(ab')2 IgG, P < 0.05]. There was also 1 1/2- to 2-fold greater anti-mitochondrial antibody F(ab')2 accumulation in the central infarct epicardium and the peripheral infarct subendocardium and subepicardium. Thus, an anti-mitochondrial antibody obtained from a patient with primary biliary cirrhosis concentrates in irreversibly damaged myocardium after experimental canine myocardial infarction. Presumably this occurs because of altered cell membrane integrity, which allows exposure of mitochondria to the anti-mitochondrial antibody. The F(ab')2 fragments of anti-mitochondrial antibodies labeled with suitable radionuclides should allow noninvasive scintigraphic detection of experimental acute myocardial infarcts.
Authors:
J T Willerson; P Kulkarni; M Stone; S E Lewis; E Eigenbrodt; F J Bonte; R W Parkey; L M Buja
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  77     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1980 Nov 
Date Detail:
Created Date:  1981-03-24     Completed Date:  1981-03-24     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  6856-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Autoantibodies*
Cell Membrane Permeability
Disease Models, Animal
Dogs
Immunoglobulin Fab Fragments
Immunoglobulin G
Microscopy, Fluorescence
Mitochondria / immunology*
Myocardial Infarction / diagnosis,  physiopathology*
Grant Support
ID/Acronym/Agency:
HL-17669/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Autoantibodies; 0/Immunoglobulin Fab Fragments; 0/Immunoglobulin G
Comments/Corrections

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