| Localization of anosmin-1a and anosmin-1b in the inner ear and neuromasts of zebrafish. | |
| | |
MedLine Citation:
|
PMID: 17064968 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Anosmin-1, encoded by the KAL-1 gene, is the protein defective in the X-linked form of Kallmann syndrome. This human developmental disorder is characterized by defects in cell migration and axon target selection. Anosmin-1 is an extracellular matrix protein that plays a role, in vitro, in processes such as cell adhesion, neurite outgrowth, axon guidance, and axon branching. The zebrafish possesses two orthologues of the KAL-1 gene: kal1a and kal1b, which encode anosmin-1a and anosmin-1b, respectively. Previous in situ hybridization studies have shown that kal1a and kal1b mRNAs are expressed in undetermined cells of the inner ear but not in neuromast cells. Using specific antibodies against anosmin-1a and anosmin-1b, we report here that both proteins are expressed in sensory hair cells of the inner ear cristae ampullaris and the lateral line neuromasts. Accumulation of these proteins was observed mainly at the level of the hair bundle and also at the cell membrane. In neuromast hair cells, immunogold scanning electronmicroscopy demonstrated that anosmin-1a and anosmin-1b were present at the surface of the stereociliary bundle. In addition, anosmin-1a, but not anosmin-1b, was detected on the track of the ampullary nerve. This is the first report of anosmin-1 expression in sensory hair cells of the inner ear and lateral line, and along the ampullary nerve track. |
| | |
Authors:
|
Sylvain Ernest; Stéphanie Guadagnini; Marie-Christine Prévost; Nadia Soussi-Yanicostas |
Related Documents
:
|
17084378 - Characterization of the calpain/calpastatin system in human hemopoietic cell lines. 9726678 - Dose-dependent response of vestibular hair cells of guinea pigs following streptomycin ... 9347798 - Expression of two ig family adhesion molecules in the murine hair cycle: dcc in the bul... 21071598 - Alström syndrome protein alms1 localizes to basal bodies of cochlear hair cells and re... 3308318 - Mechanisms of halotolerance in microorganisms. 11333118 - Novel cellular defenses against iron and oxidation: ferritin and autophagocytosis prese... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-09-10 |
Journal Detail:
|
Title: Gene expression patterns : GEP Volume: 7 ISSN: 1567-133X ISO Abbreviation: Gene Expr. Patterns Publication Date: 2007 Jan |
Date Detail:
|
Created Date: 2006-12-12 Completed Date: 2007-04-24 Revised Date: 2008-11-21 |
Medline Journal Info:
|
Nlm Unique ID: 101167473 Medline TA: Gene Expr Patterns Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 274-81 Citation Subset: IM |
Affiliation:
|
INSERM, U784, Paris, F-75005, France. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Ear, Inner / anatomy & histology, chemistry*, metabolism Extracellular Matrix Proteins / analysis*, genetics Fluorescent Antibody Technique Hair Cells, Auditory / chemistry*, metabolism, ultrastructure Immunohistochemistry Lateral Line System / chemistry*, cytology, metabolism Microscopy, Confocal Microscopy, Electron, Scanning Microscopy, Immunoelectron Nerve Tissue Proteins / analysis*, genetics Sensory Receptor Cells / chemistry*, metabolism Zebrafish / anatomy & histology, genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
|
0/Extracellular Matrix Proteins; 0/Nerve Tissue Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Glibenclamide attenuates the antiarrhythmic effect of endotoxin with a mechanism not involving K(ATP...
Next Document: Immunoaffinity chromatography of abscisic acid combined with electrospray liquid chromatography-mass...