Document Detail


Localization of angiotensin converting enzyme in the ciliary epithelium of the rat eye.
MedLine Citation:
PMID:  2551838     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Angiotensin converting enzyme (ACE, E.C. 3.14.5.1) was localized in the rat eye by immunocytochemical staining using anti-rat lung ACE monoclonal antibody, and by autoradiography using the potent ACE inhibitor [3H]captopril. Highest levels of ACE immunoreactivity and [3H]captopril binding were found in the ciliary epithelium (Bmax = 1050 fmol [3H]captopril bound/mg protein) while lower levels were present in the sclera (Bmax = 500 fmol/mg protein). Scattered regions of the choroidal epithelium were weakly immunocytochemically stained by the anti-ACE antibody. No other major sites of labeling of ACE were demonstrated. In the ciliary epithelium, ACE was confined to cells bordering on the posterior chamber of the eye, similar to its presence at the ventricular surface of the choroid plexus of the brain. These findings suggest that ACE may metabolize a peptide involved in the regulation of aqueous humor production.
Authors:
S M Strittmatter; K M Braas; S H Snyder
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Investigative ophthalmology & visual science     Volume:  30     ISSN:  0146-0404     ISO Abbreviation:  Invest. Ophthalmol. Vis. Sci.     Publication Date:  1989 Oct 
Date Detail:
Created Date:  1989-11-09     Completed Date:  1989-11-09     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7703701     Medline TA:  Invest Ophthalmol Vis Sci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2209-14     Citation Subset:  IM    
Affiliation:
Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland.
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MeSH Terms
Descriptor/Qualifier:
Animals
Autoradiography
Binding Sites
Captopril / diagnostic use,  metabolism
Ciliary Body / enzymology*,  metabolism
Epithelium / enzymology,  metabolism
Immunohistochemistry
Peptidyl-Dipeptidase A / metabolism*
Rats
Sclera / enzymology,  metabolism
Tissue Distribution
Grant Support
ID/Acronym/Agency:
DA-00266/DA/NIDA NIH HHS; MH-18501/MH/NIMH NIH HHS; NS-16375/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
62571-86-2/Captopril; EC 3.4.15.1/Peptidyl-Dipeptidase A

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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